Unknown,Transcriptomics,Genomics,Proteomics

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Differential gene expression between borderline and adenocarcinoma in ovarian cancer

ABSTRACT: Ovarian tumors of the borderline subtype (BOL) are epithelial cancers tumors with very specific features. They rarely progress towards malignancy but their evolutionary profile, although remaining local, is distinct from that of benign cancers. At the cellular level, BOL are undistinguishable from malignant tumors (cytonuclear abnormalities, abnormal mitoses, anarchical proliferation), however they never invade the stroma, which makes them clearly distinct from carcinoma. BOL tumors are of good prognosis. Even when they relapse, these tumors remain local and are, most of the time, not invasive. Thus, comparing BOL with ovarian adenocarcinomas (ADK) is of major interest to decipher potential gene alterations underlying the process of invasion, a key step of oncogenesis. In this study, 20 BOL and 19 ADK were recruited at the Gustave Roussy, frozen and analyzed by pathologists. RNA extraction followed by gene expression analysis, in association with clinical and histological data of these two groups of tumors, may provide a gene signature characterizing ovarian cancer invasion. Gene expression was performed in dual color on Agilent Whole Human Genome Oligo Microarray A-AGIL11(design 012391). Differential expression has been analysed between the 2 sets of ovary samples : 19 ADK and 20 BOL labelled in one color (Cy5) versus a pool of all samples (Cy3).

ORGANISM(S): Homo sapiens

SUBMITTER: Philippe Dessen

PROVIDER: E-MTAB-11177 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Context : Non Functioning Pituitary Adenomas (NFPAs), although typically benign, may be locally invasive. Few datas are currently available related to the molecular process of invasion in sporadic pituitary adenomas. Markers of invasiveness, important for helping the clinician in the therapeutic strategy particularly in the decision for adjuvant radiotherapy, are currently lacking. Objective: Evaluate if invasive NFPAs display a specific expression profile compared with non invasive tumors. Methods: To address this issue, we selected 40 NFPAs (38 of the gonadotroph type) and classified them as invasive (n=22) or non invasive (n=18) on the basis of MRI and surgical findings. Then we performed pangenomic analysis based on Agilent Human Whole genome Gene Expression oligonucleotide microarray (44k) Expression in order to identify genes differentially expressed between invasive and non invasive NFPA. Experiements are made in dual color with tumor samples labelled in cyanine 5 and a pool of all tumors labelled in cyanine 3.The expressions of some genes identified by microarray screening were confirmed by real-time quantitative RT-PCR. The selection of genes was made on the basis of Ingenuity networks and degree of upregulation between invasive and non invasive tumors. Moreover, some genes of interest already described in the literature were added to the analysis. Results: Prediction class analysis showed that 346 genes discriminated between invasive and non invasive NFPAs (p<0.001); 233 were up- and 113 were down-regulated between the two groups. We then tested On the basis of Ingenuity networks and degree of upregulation between invasive and non invasive tumors, a set of eight genes, including MYO5A, IGFBP5, FLT3, NFE2L1, PTTG, MMP9, NCAM and NR1H3, was tested in quantitative PCR analysis and. Results confirmed those obtained in microarrays results.analysis. At the protein level, Myosin 5A (MYO5A) demonstrated stronger immunostaining in invasive NFPAs compared to non invasive NFPAs. Conclusions: We propose a molecular signature of eight genes of grossly invasive NFPAs as compared with non invasive tumors: The product of one of these genes, MYO5A, may be a useful marker of invasive process in tumoral specimens. The role of these genes in the invasiveness process and as prognostic markers of pituitary adenomas needs to be confirmedinvestigated. Method: Microarray analyses the transcriptome of 22 invasive Non Functional Pituitary Adenomas (NFPAs) and 18 non invasive NFPAs in dual color (each sample labelled in cyanine 5 and a pool of all tumors labelled in cyanine 3).

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Differential gene expression between borderline and adenocarcinoma in ovarian cancer

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