Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of yeast isolates at different time points during fermentation

ABSTRACT: Transcriptome analysis in natural Saccharomyces cerevisiae as function of fermentation stage. Strains used were the reference strain S288C, two (06L3FF02 and 06L6FF20) isolates from the Bairrada wine region, Portugal, three (Lalvin EC-1118, Lalvin ICV D254 and AEB Fermol Rouge) wine yeast obtained commercially and one (J940047) isolate from a human patient. Fermentation was carried out in synthetic must MS300, in semi-anaerobic conditions. Cells were harvested at six time-points during fermentation: early exponential growth (T1), mid-exponential growth (T2), diauxic shift (T3), early stationary growth (T4) Mid-stationary growth (T5) and end of fermentation (T6). Hybridizations were carried out using a common reference design, using RNA obtained from S288C at T2, in dye-swap replicates, and four self-self hybridizations were performed using the common reference sample for control of the experiment background, in a total of 88 hybridizations.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Laura Carreto 

PROVIDER: E-MTAB-112 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression variability of co-regulated genes differentiates Saccharomyces cerevisiae strains.

Carreto Laura L   Eiriz Maria F MF   Domingues Inês I   Schuller Dorit D   Moura Gabriela R GR   Santos Manuel A S MA  

BMC genomics 20110420

<h4>Background</h4>Saccharomyces cerevisiae (Baker's yeast) is found in diverse ecological niches and is characterized by high adaptive potential under challenging environments. In spite of recent advances on the study of yeast genome diversity, little is known about the underlying gene expression plasticity. In order to shed new light onto this biological question, we have compared transcriptome profiles of five environmental isolates, clinical and laboratorial strains at different time points  ...[more]

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