Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TLR signalling induced by bacterial RNA vs R848

ABSTRACT: Murine dendritic cells were derived from bone-marrow of 4 mice using GM-CSF. The DC were treated with RNA from gram-positive bacteria Listeria monocytogenes packaged in DOTAP or TLR7 agonist R848. Negative controls were DOTAP with no RNA or mock.

ORGANISM(S): Mus musculus

SUBMITTER: Asa Hidmark

PROVIDER: E-MTAB-1139 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Cutting edge: TLR13 is a receptor for bacterial RNA.

Hidmark Asa A von Saint Paul Antonia A Dalpke Alexander H AH

Journal of immunology (Baltimore, Md. : 1950) 20120815 6

Bacterial RNA (bRNA) can induce cytokine production in macrophages and dendritic cells (DCs) through a previously unidentified receptor. Gene expression analysis of murine DCs showed that bRNA induced gene regulation similar to that induced by stimulation of TLR7 with R848. Although TLR7 was dispensable for cytokine induction by bRNA, TLR-associated proteins MyD88 and UNC93B were required. TLR13 is an endosomal murine TLR that has been described to interact with UNC93B with, so far, no character ...[more]

PMID: 22896636

Similar Datasets

TLR7-rsq

Project description:The spleenocytes were isolated from 3 male C57Bl/6 mice respectively (wt- dob: 7/14/09; rsq- dob: 6/27/09). The spleens were homogenized in a gentleMACSM-^Y Dissociator (Miltenyi Biotech, Germany). For red blood cell lysis the cells were resuspended in 3ml ACK lysis buffer (Sigma) and incubated for 3 min at RT. 7ml Medium (RPMI/2.5% FCS/1%PSG/1xbeta-mercaptoethanol were added. The cells were washed 1x in medium and resuspended in 5ml. 6x10^5 cells were treated with the following compounds for 4h. (final volume of 500 ul): Treatments: R0006 (5 ul/10E+06 cells + 5 ul DOTAP), DOTAP (5 ul + 5ul water x2 replicates), 10 uM R848 and DMSO. RNA extracted using QUIAShredder/RNeasy columns and stored at -80C until labeling was performed. Labeling was caried out following Agilent QUICKAMP 2-color labeling protocol. Briefly, 400 ng RNA was retrotranscribed and labeled using cyanine3 (reference) or Cyanine5 (treatment) and purified with RNeasy columns. Yield and efficiency of dye incorporation were measured at NanoDrop. Only reactions with yields >0.825 ug cRNA and yields >8 pmol dye/ug cRNA were used for hybridization. Cy5 labeling: R848, R0006 Cy3 labeling: DOTAP, DMSO

2011-03-01 | E-MEXP-3051 | biostudies-arrayexpress

CpG B and R848 induced gene expression in IRAK2 wildtype and deficient mouse macrophages

Project description:Compared to wildtype macrophages, IRAK2 deficient macrophages show higher induced gene expression in responsse to CpG B, but not R848 Manuscipt title: The dual function of IRAK2 in TLR9-mediated interfereon and proinflammatory cytokine production Bone marrow derived macrophages from wildtype and IRAK2 knockout mouse were stimulated with CpG B or R848 for 2 hours, or untreated.

2010-09-23 | E-GEOD-24264 | biostudies-arrayexpress

Microarry datasets for RAW cells stimulated with R848 in the absence or presence of apilimod or API09

Project description:Apilimod is a reported TLR pathway antagonist in clinical trial. To understand the mode of action of apilimod, we applied global microarray analysis to explore the effect of apilimod on gene expression of RAW cell in the absence of presence of R848 (TLR7 ligand). RAW cells were stimulated with R848 in the absence or presence of apilimod (1μM). mRNAs were extracted at 0h, 1h,3h, 7h, and 22h for affymetrix analysis. The inactive analog of apilimod (API09) was included as a control.

2014-02-18 | E-GEOD-22124 | biostudies-arrayexpress

Stimulation of dendritic cells with LPS and R848

Project description:Samples of monocyte derived dendritic cells (moDCs) from three healthy volunteers untreated and treated with LPS [100ng/ml] or R848 [2.5µg/ml] for different time points (0h,3h,6h,12h and 24h).

2010-12-03 | E-MTAB-448 | biostudies-arrayexpress

Transcription profiling of human dendritic cells to identify a combinatorial code by which DCs discriminate pathogens and provide (suggest) a rationale to design adjuvants for TH1 responses.

Project description:Toll like receptors (TLRs) sense microbial products and initiate adaptive immune responses by activating dendritic cells (DCs). Since pathogens may contain several agonists we asked whether different TLRs may synergize in DC activation. We report that in human and mouse DC TLR3 or TLR4 potently synergize with TLR7, TLR8 or TLR9 in the induction of selected cytokine genes. Upon synergistic stimulation, IL-12, IL-23 and Delta-4 are induced at levels 50-100 fold higher than those induced by optimal concentrations of single agonists, leading to enhanced and sustained TH1 polarizing capacity. Using microarray analysis we show that only 1.5% of the transcripts induced by single TLR agonists are synergistically regulated by combinations of TLR4 and TLR8 agonists.. These results identify a combinatorial code by which DCs discriminate pathogens and provide (suggest) a rationale to design adjuvants for TH1 responses. Series_overall_design: 3 untreated, 3 treated with LPS at 2h, 3 treated with LPS at 8h, 3 treated with R848 at 2h, 3 treated with R848 at 8h, 3 treated with LPS + R848 at 2h, 3 treated with LPS + R848 at 8h

2007-09-14 | E-GEOD-2706 | biostudies-arrayexpress

mouse BMDM poly(I:C), R848, or poly(I:C)+R848 stimulation

Project description:Gene expression kinetics for BM-DM from C57BL/6 mice challenged by poly(I:C) , R848, poly(I:C)+R848 examined at 6 time points including 0.5, 1, 2, 4, 8, 12 h.

2017-08-17 | GSE89987 | GEO

Sustained R848 treatment of Mice

Project description:PCR Array Profiling - R848 does not induce TLR-signaling related genes in TLR7-/- mice. Aim: To corroborate TLR7-dependency of R848 in mice "Triggering TLR7 in mice induces immune activation and lymphoid system disruption, resembling HIV-mediated pathology", Baenziger et al. Blood 2008

2008-09-30 | GSE12957 | GEO

mRNA expression profiles in primary microglia cells treated with R848, IFN-α, and R848+ IFN-α

Project description:Using RNA-seq, we have reported signaling crosstalk between IFN-α and R848 at the level of transcriptomes highlighting synergsitic gene expression dynamics upon different receptors signaling in microglia cells

2018-09-20 | GSE104056 | GEO

MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I independent mechanism of action

Project description:Analysis of whole mouse muscle and inguinal lymph node gene expression signature induced after 6h by in-vivo intramuscularly administration of MF59, alum, CpG, resiquimod (R848), Pam3CSK4 and DMSO and PBS controls. Analysis of splenocyte gene expression signature induced by the same treatments after 6h of incubation. MF59 and alum are licensed human vaccine adjuvants; CpG is a TLR9-agonist adjuvant; resiquimod (R848) is a TLR7/8-agonist adjuvant and Pam3CSK4 is a TLR2-agonist adjuvant.

2012-02-22 | E-MTAB-942 | biostudies-arrayexpress