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TLR signalling induced by bacterial RNA vs R848

ABSTRACT: Murine dendritic cells were derived from bone-marrow of 4 mice using GM-CSF. The DC were treated with RNA from gram-positive bacteria Listeria monocytogenes packaged in DOTAP or TLR7 agonist R848. Negative controls were DOTAP with no RNA or mock.

ORGANISM(S): Mus musculus

SUBMITTER: Asa Hidmark

PROVIDER: E-MTAB-1139 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cutting edge: TLR13 is a receptor for bacterial RNA.

Hidmark Asa A von Saint Paul Antonia A Dalpke Alexander H AH

Journal of immunology (Baltimore, Md. : 1950) 20120815 6

Bacterial RNA (bRNA) can induce cytokine production in macrophages and dendritic cells (DCs) through a previously unidentified receptor. Gene expression analysis of murine DCs showed that bRNA induced gene regulation similar to that induced by stimulation of TLR7 with R848. Although TLR7 was dispensable for cytokine induction by bRNA, TLR-associated proteins MyD88 and UNC93B were required. TLR13 is an endosomal murine TLR that has been described to interact with UNC93B with, so far, no character ...[more]

PMID: 22896636

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