Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

The impact of chemerin on transcriptomic profile of swine luteal cells during mid-luteal phase of estrous cycle

ABSTRACT: The aim of the study was to investigate the influence of chemerin on the transcriptomic profile of porcine in vitro cultured luteal cells collected during the mid-luteal phase of estrous cycle.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Sus scrofa

SUBMITTER: Jan Jastrzebski

PROVIDER: E-MTAB-11434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcription Analysis of the Chemerin Impact on Gene Expression Profile in the Luteal Cells of Gilts.

Makowczenko Karol G KG Jastrzebski Jan P JP Szeszko Karol K Smolinska Nina N Paukszto Lukasz L Dobrzyn Kamil K Kiezun Marta M Rytelewska Edyta E Kaminska Barbara B Kaminski Tadeusz T

Genes 20200612 6

Chemerin is a recently discovered adipokine that participates in the regulation of many physiological and disorder-related processes in mammals, including metabolism, inflammatory reactions, obesity, and reproduction. We investigated how chemerin affects the transcriptome profile of porcine luteal cells. The luteal cells were acquired from mature gilts. After the in vitro culturing with and without chemerin, the total RNAs were isolated and high-throughput sequencing was performed. Obtained data ...[more]

PMID: 32545672

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Project description:The equine endometrium exhibits characteristic morphological and functional changes during the estrous cycle controlled by the interplay of progesterone and estradiol. A microarray analysis of endometrial tissue samples derived from 5 time points of the estrous cycle (D0, D3, D8, D12, and D16) was performed to study the dynamics of endometrial gene expression. Endometrial biopsies were collected from five mares (Bavarian Warmblood) at the respective time points. Samples were divided and subjected to isolation of RNA for microarray analysis and analysis of tissue composition. Blood samples were collected to determine serum progesterone levels for every sample. Statistical analysis of microarray data revealed almost 10,000 differential probes corresponding to 4,996 differentially expressed genes. A cluster analysis based on gene expression profiles during the estrous cycle revealed 8 major gene expression profiles: mRNAs with highest levels 1) at D0, 2) from D0 to D3, 3) at D3, 4) from D3 to D8, 5) at D8, 6) from D8 to D12, 7) from D12 to D16, and 8) at D16. DAVID Functional Annotation Clustering revealed overrepresentation of distinct functional terms in different phases of the cycle, e.g. M-bM-^@M-^Xextracellular matrixM-bM-^@M-^Y and M-bM-^@M-^Xprotein transportM-bM-^@M-^Y during estrus, M-bM-^@M-^XDNA replication and M-bM-^@M-^Xcell cycleM-bM-^@M-^Y during early luteal phase, M-bM-^@M-^Xendoplasmic reticulumM-bM-^@M-^Y and M-bM-^@M-^Xprotein transportM-bM-^@M-^Y in the luteal phase, and M-bM-^@M-^Xinflammatory responseM-bM-^@M-^Y in the late luteal and follicular phase. Expression of selected genes of the expression clusters was validated by quantitative Real-time PCR (qPCR). This study provides new insights into global changes of equine endometrial gene expression during the estrous cycle. Equine endometrial tissue samples were collected at 5 time points during the sexual (estrous) cycle from 5 mares (5 biological replicates per time point) and analyzed with Agilent microarrays.

Dataset Information

The impact of chemerin on transcriptomic profile of swine luteal cells during mid-luteal phase of estrous cycle

Publications

Transcription Analysis of the Chemerin Impact on Gene Expression Profile in the Luteal Cells of Gilts.

Similar Datasets

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