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Transcription profiling by array of Mouse erythro-leukaemia cells during differentiation to investigate the influence of Runx1 transcription factor

ABSTRACT: We investigated the influence of Runx1 transcription factor on gene expression during the differentiation of Mouse Erythro-Leukaemia cells. Cultured cells were induced to differentiate by DMSO. Transcription profiling was done in triplicates, before and after Runx1 knockdown in both induced and non-induced MEL cells.

ORGANISM(S): Mus musculus

SUBMITTER: Vedran Franke

PROVIDER: E-MTAB-1171 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells.

van Riel Boet B Pakozdi Tibor T Brouwer Rutger R Monteiro Rui R Tuladhar Kapil K Franke Vedran V Bryne Jan Christian JC Jorna Ruud R Rijkers Erik-Jan EJ van Ijcken Wilfred W Andrieu-Soler Charlotte C Demmers Jeroen J Patient Roger R Soler Eric E Lenhard Boris B Grosveld Frank F

Molecular and cellular biology 20120716 19

RUNX1 is known to be an essential transcription factor for generating hematopoietic stem cells (HSC), but much less is known about its role in the downstream process of hematopoietic differentiation. RUNX1 has been shown to be part of a large transcription factor complex, together with LDB1, GATA1, TAL1, and ETO2 (N. Meier et al., Development 133:4913-4923, 2006) in erythroid cells. We used a tagging strategy to show that RUNX1 interacts with two novel protein partners, LSD1 and MYEF2, in erythr ...[more]

PMID: 22801375

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