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Transcriptional profiling by array of wild-type vs TNIK-/- hippocampi in adult mice

ABSTRACT: Hippocampi from adult TNIK-/- and TNIK+/+ mice were dissected out, the mRNA extracted, and hybridized to arrays. The aim was to determine the extent and nature of differential expression in the mutant, particularly those changes which might be indicative of major physiological changes.

ORGANISM(S): Mus musculus

SUBMITTER: Nathan Skene

PROVIDER: E-MTAB-1202 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TNiK is required for postsynaptic and nuclear signaling pathways and cognitive function.

Coba Marcelo P MP Komiyama Noboru H NH Nithianantharajah Jess J Kopanitsa Maksym V MV Indersmitten Tim T Skene Nathan G NG Tuck Ellie J EJ Fricker David G DG Elsegood Kathryn A KA Stanford Lianne E LE Afinowi Nurudeen O NO Saksida Lisa M LM Bussey Timothy J TJ O'Dell Thomas J TJ Grant Seth G N SG

The Journal of neuroscience : the official journal of the Society for Neuroscience 20121001 40

Traf2 and NcK interacting kinase (TNiK) contains serine-threonine kinase and scaffold domains and has been implicated in cell proliferation and glutamate receptor regulation in vitro. Here we report its role in vivo using mice carrying a knock-out mutation. TNiK binds protein complexes in the synapse linking it to the NMDA receptor (NMDAR) via AKAP9. NMDAR and metabotropic receptors bidirectionally regulate TNiK phosphorylation and TNiK is required for AMPA expression and synaptic function. TNiK ...[more]

PMID: 23035106

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