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Longitudinal clonal IgG cross-reactivity profiles of coronavirus-specific B-cells in COVID-19 patients

ABSTRACT: We investigated the kinetics, breadth, magnitude, and level of cross-reactivity of IgG antibodies against SARS-CoV-2 and heterologous seasonal (HCoV-NL63, -229E, -OC43 and -HKU1) and epidemic coronaviruses (SARS-CoV, hCoV-MERS) at the clonal level in patients with mild or severe COVID-19 as well as in disease control patients. We assessed IgG antibody reactivity to nucleocapsid and spike antigens using protein microarray. A cutoff was set at the average plus 3 times the SD of 20 nonreactive cultures with a minimum MFI of 1000.

ORGANISM(S): Homo sapiens

SUBMITTER: Gijsbert van Nierop

PROVIDER: E-MTAB-12161 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Seasonal coronavirus-specific B cells with limited SARS-CoV-2 cross-reactivity dominate the IgG response in severe COVID-19.

Aguilar-Bretones Muriel M Westerhuis Brenda M BM Raadsen Matthijs P MP de Bruin Erwin E Chandler Felicity D FD Okba Nisreen Ma NM Haagmans Bart L BL Langerak Thomas T Endeman Henrik H van den Akker Johannes Pc JP Gommers Diederik Ampj DA van Gorp Eric Cm EC GeurtsvanKessel Corine H CH de Vries Rory D RD Fouchier Ron Am RA Rockx Barry Hg BH Koopmans Marion Pg MP van Nierop Gijsbert P GP

The Journal of clinical investigation 20211101 21

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19). Little is known about the interplay between preexisting immunity to endemic seasonal coronaviruses and the development of a SARS-CoV-2-specific IgG response. We investigated the kinetics, breadth, magnitude, and level of cross-reactivity of IgG antibodies against SARS-CoV-2 and heterologous seasonal and epidemic coronaviruses at the clonal level in patients with mild or severe COVID- ...[more]

PMID: 34499051

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