Transcriptomics

Dataset Information

0

DiMNF treated NHBE cells with HCoV-OC43 Infection


ABSTRACT: The emergence of novel betacoronaviruses has posed significant financial and human health burdens, necessitating the development of appropriate tools to combat future outbreaks. In this study, we have characterized a human cell line, IGROV-1, as a robust tool to detect, propagate, and titrate betacoronavirus SARS-CoV-2 and HCoV-OC43. IGROV-1 cells can be used for serological assays, antiviral drug testing, and isolating SARS-CoV-2 variants from patient samples. Using time-course transcriptomics, we confirmed that IGROV-1 cells exhibit a robust innate immune response upon SARS-CoV-2 infection, recapitulating the response previously observed in primary human nasal epithelial cells. We performed genome-wide CRISPR knockout genetic screens in IGROV-1 cells and identified Aryl hydrocarbon receptor (AHR) as a critical host dependency factor for both SARS-CoV-2 and HCoV-OC43. Using DiMNF, a small molecule inhibitor of AHR, we observed that the drug selectively inhibits HCoV-OC43 infection but not SARS-CoV-2. Transcriptomic analysis in primary normal human bronchial epithelial cells revealed that DiMNF blocks HCoV-OC43 infection via basal activation of innate immune responses. Our findings highlight the potential of IGROV-1 cells as a valuable diagnostic and research tool to combat betacoronavirus diseases.

ORGANISM(S): Homo sapiens

PROVIDER: GSE237131 | GEO | 2023/09/19

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-09-19 | GSE237122 | GEO
2023-09-18 | GSE237121 | GEO
2022-09-30 | E-MTAB-12161 | biostudies-arrayexpress
2021-03-03 | GSE155986 | GEO
2024-03-26 | GSE262439 | GEO
2020-12-03 | PXD022017 | Pride
2023-11-01 | GSE205487 | GEO
2020-11-24 | GSE162039 | GEO
2020-11-24 | GSE162038 | GEO
2020-10-16 | E-MTAB-9638 | biostudies-arrayexpress