Mouse retina single-cell RNA-seq of WT and two Nr2e3 mutant models to explore the role of Nr2e3 in cone and rod photoreceptor differentiation
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina NovaSeq 6000
ORGANISM(S): Mus musculus
SUBMITTER: Juan Vaquerizas
PROVIDER: E-MTAB-12183 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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The linear correlation coefficients were independent of the extent to which genes were expressed in the pure cone and starburst cell groups. This suggests that A practical use of the finding that amplification is linear is to separate the transcriptome of a cell group that contains cells from different types into its cell-type components. Assuming, for example, a cell group (Group A) containing a mixture of two cell types, Type 1 and Type 2, as well as a futher cell group (Group B) containing only Type 1 (Supplementary Figure 10). We wish to determine the transcriptome of Type 2 but we have no cell group (Group C) that contains Type 2 only. Or more generally, assume a cell group (Group A) containing a mixture of cell types, Type 1, Type 2…Type N, as well as a further cell group (Group B) containing only Type 1. We wish to determine the mixed transcriptome of Group C that contains Type 2….Type N, without Type 1. This separation of cell-type transcriptomes is useful for two reasons. 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