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Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of mouse brain tissue after exposure to p-cresol sulfate

ABSTRACT: In vivo work done to determine how p-cresol glucuronide (pCS) affects integrity of the blood-brain barrier. Wild-type male C57Bl/6 mice were injected with saline or pCS, and killed 2 h after injection. Mice were transcardially perfused with 0.9% saline at 4 °C to remove circulating blood, and brains were removed and collected into RNAlater. Whole brain total RNA was extracted using a PureLink RNA Mini Kit. RNA samples (n=5 pCS, n=5 control) were sent to Macrogen Inc. (Republic of Korea) where they were subject to quality checks (RIN analysis), library prep and sequencing [TruSeq Stranded mRNA LT Sample Prep Kit; paired-end (2x 100 nt) sequencing; Illumina HiSeq 4000]. Only R1 (forward strand) reads were analysed and have been uploaded to ArrayExpress.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER:

PROVIDER: E-MTAB-12326 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP142392

REPOSITORIES: biostudies-arrayexpress

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RNA-seq of mouse brain tissue after exposure to p-cresol glucuronide

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