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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome of human HT1080 cells treated with purified Salmonella Typhi toxin

ABSTRACT: Aim of the experiment: To examine changes in gene expression in HT1080 fibroblasts following treatment with purified recombinant wild-type typhoid toxin (TOX) from Salmonella Typhi relative to a mutant H160Q variant lacking DNase activity (TOX-H160Q). Experimental workflow: HT1080 were seeded one day before intoxication into T75 tissue culture flasks for a 30% confluency in DMEM supplemented with 10% FBS and PenStrep. The next day, TOX (5 ng/ml) or negative control TOX-H160Q (5 ng/ml) was incubated for 2h with cells followed by three washes with PBS and a 48h chase in DMEM supplemented with 10% FBS and PenStrep. After 48 h, cells were collected by trypsinisation. RNA was isolated and samples analysed using human Clariom S assay (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems, Thermo Fisher Scientific).

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Humphreys

PROVIDER: E-MTAB-12333 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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