Project description:RNA was extracted from FFPE samples by manual microdissection of the tumor zones. RNAseq libraries were perfomed using Lexogen's QuantSeq (reverse) 3' kit
Project description:H9 human ESCs (H9 line) were cultured in BMP4 cultured medium on Matrigel-coated plates. Colonies were passaged for maintanence by Gentle Cell Dissociation Reagent
Project description:We used cerebral organoids generated from wildtype and CHD8 +/- human ES cells to study the effects of CHD8, one of the top ASD risk genes, on early cortical development. CHD8 +/- hESC were generated using the CRISPR/Cas9 system to create a deletion within the helicase domain. Cerebral organoids were generated according to the protocol from Lancaster et al 2013 with minor modifications.
Project description:Lumpy Skin Disease (LSD) is an endemic viral infection in Africa, newly emerging in Europe and becoming a global threat. There is host variation in response to infections in field studies and under carefully controlled artificial infections. It’s sometimes around 50% in clinical trials. This study aims to understand the mechanisms underlying this diversity and find the determinants to distinguish between susceptible (recovered) and resistant (non-recovered) animals through Differential Gene Expression (DGE) analysis. Five approximately 6-month-old male Holstein bulls were experimentally infected with LSDV. RNA sequencing was done for whole blood samples collected five days prior (pre-infection) to 3, 7, and 15 days after the infection (post-infection) challenge. After the challenge, two animals recovered, and three did not.