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RIL-seq of Klebsiella pneumoniae SGH10 grown in LB or DMEM medium

ABSTRACT: RIL-seq experiment allows identification of pairs of interacting RNA molecules while bound to the Hfq protein. SGH10 bacteria were grown under DMEM (Dulbecco's Modified Eagle Medium) and LB (Lysogeny broth) conditions. RIL-seq was performed as previously described in Melamed et al. 2018 with adaptation of the computational analysis to the SGH10. It was applied to three biological replicates, for each growth condition. Libraries were sequenced by paired-end sequencing using Nextseq 500 Sequencer (Illumina). sequence fragments were mapped to SGH10 which contains a chromosome NZ_CP025080.1 and a plasmid NZ_CP025081.1. RIL-seq derived sequence fragments that mapped to the same locus or to two different loci were defined as single and chimeric fragments respectively. Chimeric fragments that appeared statistically significantly more than expected at random (S-chimeras) were considered as representing putative interacting RNAs.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Klebsiella pneumoniae

SUBMITTER: Liron Argaman

PROVIDER: E-MTAB-13342 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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