Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Illumina 850K EPIC methylation arrays of blood, subcutaneous and omental visceral adipose tissue from two-step surgical approach

ABSTRACT: Genome wide DNA methylation in blood, subcutaneous and omental visceral adipose tissue from two-step surgical approach (N=9) was analysed in patients with severe obesity using Illumina 850K EPIC technology before and after metabolic surgery (Leipzig Obesity BioBank (LOBB) cohort). Additionally, a validation blood cohort of patients with obesity undergoing metabolic surgery was analyzed for results validation.

ORGANISM(S): Homo sapiens

SUBMITTER: Anne Hoffmann

PROVIDER: E-MTAB-13564 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human adipose tissue pre and post bariatric surgery

Project description:Adipose tissue before and after bariatric surgery (BPD/DS)-Pilot study using AB1700 microarrays. Subcutaneous abdominal adipose tissue pre and post bariatric surgery (BPD/DS).

2010-06-10 | E-TABM-864 | biostudies-arrayexpress

Transcription profiling of human adipose tissue cells before and after bariatric surgery (BPD/DS)

Project description:Comparison of subcutaneous abdominal adipose tissue before and after biliopancreatic diversion with duodenal switch (BPD/DS), and versus subcutaneous abdominal adipose tissue from lean, healthy subjects undergoing hernia repair surgery

2010-06-10 | E-TABM-862 | biostudies-arrayexpress

Expression data from human adipose tissue

Project description:Obesity is a risk factor for numerous metabolic disorders; however, not all obese individuals are prone to insulin resistance. The central aim of this study was to identify molecular pathways directly related to insulin resistance independent of BMI in obesity. We sought to determine the gene expression signature of adipose tissue in a body mass index (BMI)-matched obese cohort of patients that are either insulin sensitive or insulin resistant. We determined the global gene expression signatures of omental and subcutaneous adipose tissue samples obtained from insulin-sensitive obese and insulin-resistant obese patients undergoing gastric bypass surgery.

2009-04-24 | E-GEOD-15773 | biostudies-arrayexpress

Gene methylation profiles in visceral adipose tissue of obese men with and without the metabolic syndrome

Project description:Evaluate differences in gene methylation levels between obese men with and without the metabolic syndrome Visceral adipose tissue from obese men with the metabolic syndrome (MetS+, N=7) vs. obese men without the metabolic syndrome (MetS-, N=7)

2014-02-07 | E-GEOD-54776 | biostudies-arrayexpress

The inflammasome is a central player in the induction of obesity and insulin resistance

Project description:Inflammation plays a key role in the pathogenesis of obesity. Chronic overfeeding leads to macrophage infiltration in the adipose tissue, resulting in pro-inflammatory cytokine production. Both microbial and endogenous danger signals trigger assembly of the intracellular innate immune sensor Nlrp3 [NLR family, pyrin domain containing 3] resulting in caspase-1 activation and production of pro-inflammatory cytokines interleukin (IL)-1beta and IL-18. Here, we showed that mice deficient in Nlrp3, ASC [apoptosis-associated speck-like protein containing a CARD; a.k.a PYCARD (PYD and CARD domain containing)] and caspase-1 were resistant to the development of high fat diet-induced obesity, which correlated with protection from obesity-induced insulin resistance. Detailed metabolic and molecular phenotyping demonstrated that the inflammasome controls energy expenditure and adipogenic gene expression during chronic overfeeding. These findings reveal a critical function of the inflammasome in obesity and insulin resistance and suggest inhibition of the inflammasome as a potential therapeutic strategy. Keywords: Expression profiling by array Wild-type (WT), ASC-null and Casp1-null mice were subjected to high fat diet feeding for 16 weeks. After the diet intervention period, the animals were killed and epididymal white adipose tissue was removed. Total RNA was isolated and subjected to gene expression profiling.

2011-08-20 | E-GEOD-25205 | biostudies-arrayexpress

The Epigenome of Spermatozoa is Affected in Obesity and Dynamically Changes after Bariatric Surgery [Lean/Obese MNase]

Project description:Obesity is a heritable disorder, with children of obese fathers at higher risk of developing obesity. Â Environmental factors epigenetically influence somatic tissues, but the contribution of these factors to the establishment of epigenetic patterns in human gametes is unknown. Here, we hypothesized that weight loss remodels the epigenetic signature of spermatozoa in human obesity. Comprehensive profiling of the epigenome of sperm from lean and obese men showed similar histone positioning, but small non-coding RNA expression and DNA methylation patterns were markedly different. In a separate cohort of morbidly obese men, surgery-induced weight loss was associated with a dramatic remodeling of sperm DNA methylation, notably at genetic locations implicated in the central control of appetite. Our data provide evidence that the epigenome of human spermatozoa dynamically changes under environmental pressure, and offers Â insight into how obesity may propagate metabolic dysfunction to the next generation. Examination of the DNA methylation status, histone retention and sncRNA expression of the semen of 13 lean and 10 obese individuals; as well as the DNA methylation status of the semen of 6 obese men undergoing Roux-en-Y GBP surgery, at three time points: approximately 1 week before, 1 week after and 1 year after the surgery.

2015-12-04 | E-GEOD-74426 | biostudies-arrayexpress

Transcription profiling of human adipose tissue using an expanded patient cohort

2010-04-07 | E-GEOD-20950 | biostudies-arrayexpress

Illumina methylation EPIC arrays of human blood at baseline and after 18 months of dietary interventions

Project description:To highlight molecular mechanisms underlying the beneficial metabolic effects of a Mediterranean (MED) diet, a MED diet enriched in polyphenols and reduced in red/processed meat (greenMED), and a diet with healthy dietary guidelines (HDG), we analyzed their effects on the methylome in the 18-month DIRECT PLUS randomized controlled trial. The methylation arrays contains individuals from the three intervention arms (MED, greenMED, HDG) for baseline (T0). We will gladly provide further metadata upon request.

2023-07-26 | E-MTAB-12527 | biostudies-arrayexpress

Methylation of different human adipose tissues

Project description:Characterization of human epiploic adipose tissue (AT), compared to other AT: omental, mesenteric and subcutaneous. AT was surgically removed, collected and methylome as well as proteome and transcriptome was analyzed. For 12 donors, DNA methylation was analyzed for all 4 aforementioned AT types, while 2 donors contributed one and 3 types, resp. Donors showed obesity, but were either insulin sensitive or insulin resistant.

2021-11-01 | E-MTAB-10999 | biostudies-arrayexpress

Transcription profiling of white adipose tissue from Lldlr-/- and Lldlr-/-Mif-/- mice

Project description:MIF deficiency reduces chronic inflammation in white adipose tissue and impairs development of insulin resistance, glucose intolerance and associated atherosclerotic disease

2009-06-19 | E-TABM-524 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data