Identifying crossover locations in an Arabidopsis thaliana Col x Ler F2 population using Nanopore-seq and COmapper
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ABSTRACT: Genomic DNA from 55 wild type Col x Ler F2 individuals was extracted using the CTAB method. Equal amounts of DNA from these 55 plants were pooled into two groups (pool 1 = 4 plants; pool 2 = 51 plants), and nine micrograms of gDNA from each pool was used to generate Nanopore sequencing libraries with the Ligation Sequencing Kit V14 (Nanopore, SQK-LSK114). The libraries were sequenced independently using PromethION (BGI, Hong Kong).
INSTRUMENT(S): PromethION
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Kyuha Choi
PROVIDER: E-MTAB-14367 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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