Identifying crossover locations in an Arabidopsis thaliana Col x Ler F2 population using Nanopore-seq and COmapper

ABSTRACT: Genomic DNA from 55 wild type Col x Ler F2 individuals was extracted using the CTAB method. Equal amounts of DNA from these 55 plants were pooled into two groups (pool 1 = 4 plants; pool 2 = 51 plants), and nine micrograms of gDNA from each pool was used to generate Nanopore sequencing libraries with the Ligation Sequencing Kit V14 (Nanopore, SQK-LSK114). The libraries were sequenced independently using PromethION (BGI, Hong Kong).

INSTRUMENT(S): PromethION

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kyuha Choi 

PROVIDER: E-MTAB-14367 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress