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Transcription profiling by array of 376 budding yeast wildtypes for assessing day-to-day variance

ABSTRACT: Contains a colection of budding yeast wildtypes grown in parallel to 1,484 budding yeast deletion mutants to assess day-to-day variance. Related to E-MTAB-1384, E-MTAB-1383, E-TABM-773, E-TABM-984, E-MTAB-1351 and E-MTAB-1352

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Patrick Kemmeren

PROVIDER: E-MTAB-1461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Large-scale genetic perturbations reveal regulatory networks and an abundance of gene-specific repressors.

Kemmeren Patrick P Sameith Katrin K van de Pasch Loes A L LA Benschop Joris J JJ Lenstra Tineke L TL Margaritis Thanasis T O'Duibhir Eoghan E Apweiler Eva E van Wageningen Sake S Ko Cheuk W CW van Heesch Sebastiaan S Kashani Mehdi M MM Ampatziadis-Michailidis Giannis G Brok Mariel O MO Brabers Nathalie A C H NA Miles Anthony J AJ Bouwmeester Diane D van Hooff Sander R SR van Bakel Harm H Sluiters Erik E Bakker Linda V LV Snel Berend B Lijnzaad Philip P van Leenen Dik D Groot Koerkamp Marian J A MJ Holstege Frank C P FC

Cell 20140401 3

To understand regulatory systems, it would be useful to uniformly determine how different components contribute to the expression of all other genes. We therefore monitored mRNA expression genome-wide, for individual deletions of one-quarter of yeast genes, focusing on (putative) regulators. The resulting genetic perturbation signatures reflect many different properties. These include the architecture of protein complexes and pathways, identification of expression changes compatible with viabili ...[more]

PMID: 24766815

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