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Unknown,Transcriptomics,Genomics,Proteomics

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Array_devplast_feb2012

ABSTRACT: Limited nutrient availability during development puts individuals at risk to develop complications later in life. Central in this early-life stress paradox lies developmental plasticity, a poorly understood mechanism that responds to environmental cues from early to late developmental stages. In this study, we aim to introduce the zebrafish (Danio rerio) as a model to study the early-developmental responses to reduced nutrient availability and their outcome. To reduce nutrient availability, we partially remove the yolk during embryogenesis. Around 5 hours post-fertilization, from 3 batches of embryos, we removed ~30% of the yolk (YD samples) or sham-punctured embryos (SP) with a Hamilton syringe system. At 2 days post-fertilization, we collected RNA from whole embryos and obtained transcriptome profiles by microarray hybridization.

ORGANISM(S): Danio rerio

SUBMITTER: Sam Linsen

PROVIDER: E-MTAB-1605 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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