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LIN28 eRIP in Differentiating hESCs

ABSTRACT: Excluded-volume-effect-optimized RNA-IP (eRIP) of LIN28-associated mRNAs in H1 hESCs. H1s are induced to differentiate into either trophoblast (TE) or neural (N) lineages, and LIN28 eRIP performed at 0hr (Undifferentiated), 12hr TE, 24hr TE and 24hr N

ORGANISM(S): Homo sapiens

SUBMITTER: Shen Mynn Tan

PROVIDER: E-MTAB-1652 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation.

Tan Shen Mynn SM Altschuler Gabriel G Zhao Tian Yun TY Ang Haw Siang HS Yang Henry H Lim Bing B Vardy Leah L Hide Winston W Thomson Andrew M AM Lareu Ricky R RR

Nucleic acids research 20140523 12

LIN28 function is fundamental to the activity and behavior of human embryonic stem cells (hESCs) and induced pluripotent stem cells. Its main roles in these cell types are the regulation of translational efficiency and let-7 miRNA maturation. However, LIN28-associated mRNA cargo shifting and resultant regulation of translational efficiency upon the initiation of differentiation remain unknown. An RNA-immunoprecipitation and microarray analysis protocol, eRIP, that has high specificity and sensit ...[more]

PMID: 24860167

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