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Human Embryonic Stem Cells Polysomes

ABSTRACT: H1s are induced to differentiate into the trophoblast (TE) lineage, and polysome analysis performed at 0hr (Undifferentiated), 12hr TE and 24hr TE. The whole cell extract (WCE) is a cytoplasmic lysate of the cell . G1-G4 represent RNAs that were found in different fractions of a sucrose gradient, which represents monosomes, polysomes, etc. Thus the WCE is used as the denominator so that for a particular transcript, we can express G1, for example, as a fraction of the total RNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Shen Mynn Tan

PROVIDER: E-MTAB-1653 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation.

Tan Shen Mynn SM Altschuler Gabriel G Zhao Tian Yun TY Ang Haw Siang HS Yang Henry H Lim Bing B Vardy Leah L Hide Winston W Thomson Andrew M AM Lareu Ricky R RR

Nucleic acids research 20140523 12

LIN28 function is fundamental to the activity and behavior of human embryonic stem cells (hESCs) and induced pluripotent stem cells. Its main roles in these cell types are the regulation of translational efficiency and let-7 miRNA maturation. However, LIN28-associated mRNA cargo shifting and resultant regulation of translational efficiency upon the initiation of differentiation remain unknown. An RNA-immunoprecipitation and microarray analysis protocol, eRIP, that has high specificity and sensit ...[more]

PMID: 24860167

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