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RNA-sequencing DE Spd (humerus)

ABSTRACT: RNA-sequencing profiling analysis was performed on TS23 humerus tissue from muscle-less mutant (splotch-delayed) mice and compared to control littermates. The purpose of this profiling analysis was to identify differentially expressed genes between muscle-less and control embryonic (TS23) humerus tissue, in order to determine mechanosensitive genes that impact skeletal development.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Mus musculus

SUBMITTER: Rebecca Rolfe

PROVIDER: E-MTAB-1746 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of mechanosensitive genes during skeletal development: alteration of genes associated with cytoskeletal rearrangement and cell signalling pathways.

Rolfe Rebecca A RA Nowlan Niamh C NC Kenny Elaine M EM Cormican Paul P Morris Derek W DW Prendergast Patrick J PJ Kelly Daniel D Murphy Paula P

BMC genomics 20140120

<h4>Background</h4>Mechanical stimulation is necessary for regulating correct formation of the skeleton. Here we test the hypothesis that mechanical stimulation of the embryonic skeletal system impacts expression levels of genes implicated in developmentally important signalling pathways in a genome wide approach. We use a mutant mouse model with altered mechanical stimulation due to the absence of limb skeletal muscle (Splotch-delayed) where muscle-less embryos show specific defects in skeletal ...[more]

PMID: 24443808

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