Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next-Generation Sequencing was used to conduct a quantitative analysis of Wild Type (Ctrl) and Stat3 KO (Test) long bone transcriptomes.

ABSTRACT: RNA samples were isolated shaft of the humerus bone of E16.5 embryos. A total of six, three ctrl & three test samples were used. RNASeq analyses were conducted to identify the differentially expressed genes upon Stat3 loss of function during long bone development. Total RNA was isolated from the long bone of E16.5 embryos of the indicated genotypes.

ORGANISM(S): Mus musculus

PROVIDER: GSE159184 | GEO | 2021/07/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Yap/Taz negatively regulate bone angiogenesis and osteogenesis

Project description:In long bone, the metaphyseal region is less hypoxic than the bone marrow (bone shaft). To study the regional differences of vasculatures, we isolated endothelial cells (ECs) expressing membrane-anchored mTomato protein and nuclear H2B-GFP from Cdh5-mT/nG transgenic reporter femoral metaphysis and diaphyseal bone marrow cavity by fluorescence-activated cell sorting (FACS) at high purity for RNA sequencing (RNA-seq). Next, to study the Hippo signaling in bone ECs, we performed RNA-seq analysis of freshly isolated bone ECs from Cdh5Cre-ERT2-positive Yap1/Taz double floxed or Cdh5Cre-ERT2-positive Lats2 floxed allele and their Cre-negative corresponding littermate controls.

2020-01-15 | GSE102181 | GEO

mRNA expression in rat proximal femoral growthplate after mid-shaft fracture

2006-03-31 | GSE3298 | GEO

Lymphatic endothelial cell identity is controlled by a GATA2 bound enhancer (Microarray)

Project description:This study investigates the transcriptome of primary dermal lymphatic endothelial cells compared with blood vascular endothelial cells using samples isolated from wildtype embryos at defined points (E14.5, E16.5 and E18.5) during mouse embryonic development.

2022-11-21 | GSE184045 | GEO

Chicken Tibial Bone

Project description:RNA extracted from 70 week old mid shaft tibial bone (including marrow) of white leghorn hens (female) varying in bone strength

2014-08-25 | E-MTAB-2737 | biostudies-arrayexpress

Transcription profiling of rat proximal femoral growthplate after mid-shaft fracture

Project description:Mid-shaft fracture stimulates bone lengthening by increasing linear growth at the growthplate. This project studied changes in mRNA in the proximal growthplate after a mid-shaft fracture in a rat model. Experiment Overall Design: Study of rat proximal femoral growthplate after mid-shaft fracture in 4 week old Sprague-Dawley female rats. RNA from two rats were pooled for each sample. Proximal femoral growthplate was studied from the fractured femora and the intact contralateral femora.

2008-06-12 | E-GEOD-3298 | biostudies-arrayexpress

Next-Generation Sequencing was used to conduct a quantitative analysis of Wild Type (Ctrl) and Stat3 KO (Test) long bone transcriptomes.

Project description:Next-Generation Sequencing was used to conduct a quantitative analysis of Wild Type (Ctrl) and Stat3 KO (Test) long bone transcriptomes.

| PRJNA667910 | ENA

Collagen10-Cre;Rosa26-tdTomato e16.5 single cell RNA-sequencing

Project description:In order to assess the descendants of hypertrophic chondrocytes, we utilized Collagen10-Cre;Rosa26-tdTomato mouse total bone isolated at e16.5 by Collagenase II digestion after mechanical digestion and soft tissue removal. After sequencing and downstream analysis using Seurat, we observed clusters of cells with gene profiles matching classically defined chondrocytes, skeletal stem and progenitor cells (SSPCs), and osteoblasts. Trajectory analysis reveals that the SSPCs lie intermediate to the transition of chondrocyte to osteoblast. We conclude that hypertrophic chondrocytes dedifferentiate to this progenitor stage before further differentiation.

2022-02-16 | GSE190616 | GEO

Microarray mRNA expression profiling of E16.5 foetal gonocytes

Project description:Gonocytes were FACS sorted from testes of E16.5 Miwi2 Tom/+ embryos using the tdTomato reporter knocked into the Miwi2 locus. RNA was isolated from sorted cells and transcript expression analyzed by microarray measurement.

2020-06-18 | E-MTAB-7985 | biostudies-arrayexpress

Retroviral Sox17 over-expression adult hematopoietic stem/progenitor cells microarray

Project description:The transcription factor SOX17 is expressed by fetal, but not adult hematoipoietic stem cells (HSCs), and is required for the maintenance of fetal and neonatal, but not adult, HSCs. In the current study we show that ectopic expression of Sox17 in adult HSCs and transiently reconstituting multipotent progenitors was sufficient to confer increased self-renewal potential and the expression of fetal HSC genes including fetal HSC surface markers. To assess the mechanisms by which ectopic Sox17 expression in adult hematopoietic progenitors increased self-renewal potential and conferred fetal HSC properties, we compared the gene expression profiles of E16.5 fetal liver HSCs, young adult bone marrow HSCs, young adult bone marrow CD48+LSK cells, and Sox17-expressing CD48+LSK cells isolated from mice that had been transplanted with MSCV-Sox17-infected bone marrow cells 12 weeks earlier. Total RNA (~5ng) was isolated from 3 independent, freshly isolated aliquots of 10,000 E16.5 fetal liver HSCs, 10,000 fetal liver CD48+LSK cells, 10,000 adult bone marrow HSCs, 10,000 adult bone marrow CD48+LSK cells, 10,000 Sox17-expressing CD48+LSK cells isolated from primary recipients 12 weeks after transplantation of MSCV-Sox17-infected bone marrow cells. Purified RNA was reverse transcribed and amplified using the WT-Ovation™ Pico RNA Amplification system (NuGEN Technologies) following the manufacturer’s instructions. Sense strand cDNA was generated using WT-Ovation™ Exon Module (NuGEN), then fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN). 2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.

2011-07-14 | E-GEOD-30444 | biostudies-arrayexpress

Transcription profiling of fetal livers of UPF2 null mice

Project description:Deletion of the NMD component UPF2 in fetal liver. Upf2flox/+ females were mated to Upf2flox/+; Alfp-Cre males. Fetal livers were isolated from Upf2+/+; Alfp-Cre (WT) and Upf2flox/flox; AlfpCre (UPF2 null) E16.5 and E18.5 embryos.

2010-06-17 | E-MEXP-2248 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data