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NcRNA termination in Saccharomyces cerevisiae

ABSTRACT: Nuclear depletion of the essential transcription termination factor Nrd1 in Saccharomyces cerevisiae was studied using a combination of RNA-Seq, ChIP-Seq of Pol II and PAR-CLIP of Nrd1. The drug rapamycin induces the formation of a ternary complex between a protein of interest, the drug and the small subunit of the ribosome (both proteins are genetically engineered). The small ribosome subunit is transported out of the nucleus. therefore the protein of interest can be depleted from nucleus upon treatment with rapamycin.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Daniel Schulz

PROVIDER: E-MTAB-1766 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome surveillance by selective termination of noncoding RNA synthesis.

Schulz Daniel D Schwalb Bjoern B Kiesel Anja A Baejen Carlo C Torkler Phillipp P Gagneur Julien J Soeding Johannes J Cramer Patrick P

Cell 20131107 5

Pervasive transcription of eukaryotic genomes stems to a large extent from bidirectional promoters that synthesize mRNA and divergent noncoding RNA (ncRNA). Here, we show that ncRNA transcription in the yeast S. cerevisiae is globally restricted by early termination that relies on the essential RNA-binding factor Nrd1. Depletion of Nrd1 from the nucleus results in 1,526 Nrd1-unterminated transcripts (NUTs) that originate from nucleosome-depleted regions (NDRs) and can deregulate mRNA synthesis b ...[more]

PMID: 24210918

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