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Unknown,Transcriptomics,Genomics,Proteomics

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Myeloid lineage switch in pro-B cells generates novel macrophage subsets during homeostasis and inflammation

ABSTRACT: Here we demonstrate for the first time evidence of lineage switch from B-1 B lymphocyte to a macrophage , or so-called transdifferentiation, occurring in mature cells in vivo. Specifically, using transgenic B6.Mb1-iCre/Rosa26-YFP mice, in which YFP expression is restricted exclusively to B cell lineage, we discovered that a significant portion of tissue-resident macrophages in peritoneum, pleural cavity and intestine in steady state are positive for YFP. B cell origin of YFP+ macrophages was confirmed by next-generation sequencing of genomic DNA locus encoding immunoglobulin heavy chain genes. Eliciting a self-resolving zymosan peritonitis showed that during inflammation a subset of B-1 B cells gives rise to inflammation-induced macrophages. The aim of this study was to perform transcriptome analysis of B-1 B cell-derived macrophages (B-1/macrophages) from the naM-ove and inflamed murine peritoneum and compare them to already established populations of naM-ove tissue-resident macrophages, inflammation-experienced resident (yolk-sac-derived) macrophages and inflammation-induced (monocyte-derived) macrophages.

ORGANISM(S): Mus musculus

SUBMITTER: Dan Frampton

PROVIDER: E-MTAB-1878 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Alternatively activated macrophages derived from monocytes and tissue macrophages are phenotypically and functionally distinct

Project description:Macrophages adopt an alternatively activated phenotype (AAM) when activated by IL-4. While these AAMs can be derived from local proliferation of resident tissue macrophages or recruited inflammatory monocytes, it is not known whether these different AAMs are phenotypically and functionally distinct. Using microarray analysis of gene expression, we demonstrated that while both monocyte- and tissue-derived AAM expressed high levels of Arg1, Chi3l3 and Retnla, only monocyte-derived AAM upregulated Raldh2 and PD-L2. The distinction between monocyte- and tissue-derived macrophages can, therefore, be made using different phenotypic markers. Gene expression profiling analysis with whole genome microarray of tissue-derived and monocyte-derived macrophages, induced using IL-4c and/or 4% thioglycollate.

2014-02-05 | E-GEOD-54679 | biostudies-arrayexpress

MicroRNA expression in thioglycollate and alternatively activated

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2012-08-07 | E-GEOD-35047 | biostudies-arrayexpress

Klf4 expression in conventional dendritic cells is required for T helper 2 cell responses

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2012-11-01 | E-MTAB-995 | biostudies-arrayexpress

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2011-01-08 | E-MEXP-2545 | biostudies-arrayexpress

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2020-01-06 | E-MTAB-8140 | biostudies-arrayexpress

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2018-02-01 | E-MTAB-5538 | biostudies-arrayexpress

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2016-07-01 | E-GEOD-70520 | biostudies-arrayexpress

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