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Transcriptional changes in cells upon exposure and during internalisation of aggregating peptides

ABSTRACT: The goal of the study is the analysis of the transcriptional adaptation of cultured cells to the exposure and uptake of peptide aggregates of different sizes. Synthetic aggregating peptides with low and high aggregation propensities that form small (<500 nm, Peptide PepS) versus large (>1 µm, PepL) aggregate size distributions were designed. We found that soluble peptides and small aggregates (less than 500 nm diameter) were taken up by non-specific endocytosis as part of the fluid phase and travelled through the endosomal compartment to lysosomes. By contrast, aggregates bigger than 1 µm in diameter, which could not be internalized along with the fluid phase, were taken up through a mechanism dependent of cytoskeletal reorganization and membrane remodelling with the morphological hallmarks of phagocytosis. The microarray analysis aims to determine what's the impact of these different mechanisms of aggregate endocytosis on the transcriptional activity of the cell.

ORGANISM(S): Homo sapiens

SUBMITTER: Joke Allemeersch

PROVIDER: E-MTAB-1982 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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