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Effect of PTFGRN Expression on the Proteomic Profile of A431 Cells and Determination of the PTGFRN Interactome

ABSTRACT: The exact molecular mechanisms by which PTGFRN influences cancer progression are still unknown. Our laboratory set out to investigate how PTGFRN knockdown affected the expression of other proteins. We also carried out co-immunoprecipitation experiments using a monoclonal anti-PTGFRN antibody. We employed mass spectrometry-based proteomics for both experiments to identify proteins that were associated with PTGFRN.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mehari Weldemariam

LAB HEAD: Maureen A. Kane

PROVIDER: PXD048092 | Pride | 2024-06-22

REPOSITORIES: Pride

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Effect of PTFGRN Expression on the Proteomic Profile of A431 Cells and Determination of the PTGFRN Interactome.

Marquez Jorge J Weldemariam Mehari M MM Dong Jianping J Hayashi Jun J Kane Maureen A MA Serrero Ginette G

ACS omega 20240315 12

Prostaglandin F2 receptor negative regulator (PTGFRN) is a transmembrane protein whose expression has been previously implicated in cancer metastasis. However, the exact molecular mechanisms by which PTGFRN influences cancer progression are still unknown. As such, our laboratory set out to investigate how PTGFRN knockdown affected the expression of other proteins. We also carried out coimmunoprecipitation experiments using a monoclonal anti-PTGFRN antibody. We employed mass spectrometry-based pr ...[more]

PMID: 38559916

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