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Ago2-bound ShortRnas in HCT116 cell line and Dicer_hypomorphic(DICER_Ex5 cells) HCT116 cell line

ABSTRACT: DICER_Ex5 cells were created by disrupting exon 5 of the human Dicer gene using an AAV targeting construct, thereby interrupting a well conserved segment of the N-terminal helicase domain while sparing the RNase III domains. In DICER_Ex5 cells, Dicer is expressed at a level lower than the wild type. This experiment started with RIP-anti-Ago2 pull-down, followed by high throughput sequencing analysis in wild type and Dicer-hypomorphic HCT116 cell lines.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Homo sapiens

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PROVIDER: E-MTAB-2056 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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