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MiR-522 Pulldown-seq in MDA-MB-468

ABSTRACT: Identifying miRNA-regulated genes is key to understanding miRNA function. However, many miRNA recognition elements (MREs) do not follow canonical seed base-pairing rules, making identification of bona fide targets challenging. Here, we apply an unbiased sequencing-based systems approach to characterize miR-522, a member of the oncogenic primate-specific chromosome 19 miRNA cluster, highly expressed in poorly differentiated cancers. To identify miRNA targets, we sequenced full-length transcripts captured by a biotinylated miRNA mimic (this dataset). Within these targets, mostly non-canonical MREs were identified by sequencing RNase-resistant fragments.

INSTRUMENT(S): Ion Torrent PGM

ORGANISM(S): Homo sapiens

SUBMITTER: Shen Mynn Tan

PROVIDER: E-MTAB-2112 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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