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Transcription profiling of wild type, gis1 knockout, rph1 knockout, gis1rph1 double knockout, rph1-H235A mutant and gis1 knockout/rph1-H235A mutant yeast at different growth phases

ABSTRACT: Gene regulatory significance of the JmjC domain H235A point mutation in the yeast transcription factor Rph1, in log and post-diauxic shift (pds) phase

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Niklas Nordberg

PROVIDER: E-MTAB-2147 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The histone demethylase activity of Rph1 is not essential for its role in the transcriptional response to nutrient signaling.

Nordberg Niklas N Olsson Ida I Carlsson Mattias M Hu Guo-Zhen GZ Orzechowski Westholm Jakub J Ronne Hans H

PloS one 20140707 7

Rph1 and Gis1 are two related yeast zinc finger proteins that function as downstream effectors in the Ras/PKA, TOR and Sch9 nutrient signaling pathways. Both proteins also contain JmjC histone demethylase domains, but only Rph1 is known to be an active enzyme, demethylating lysine 36 of histone H3. We have studied to what extent the demethylase activity of Rph1 contributes to its role in nutrient signaling by performing gene expression microarray experiments on a yeast strain containing a cataly ...[more]

PMID: 24999627

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