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B16-F10 RNA-Seq

ABSTRACT: RNA-Seq of B16-F10 mouse melanoma cell line and gene expression profiling

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Patrick Sorn

PROVIDER: E-MTAB-2271 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Exploiting the mutanome for tumor vaccination.

Castle John C JC Kreiter Sebastian S Diekmann Jan J Löwer Martin M van de Roemer Niels N de Graaf Jos J Selmi Abderraouf A Diken Mustafa M Boegel Sebastian S Paret Claudia C Koslowski Michael M Kuhn Andreas N AN Britten Cedrik M CM Huber Christoph C Türeci Ozlem O Sahin Ugur U

Cancer research 20120111 5

Multiple genetic events and subsequent clonal evolution drive carcinogenesis, making disease elimination with single-targeted drugs difficult. The multiplicity of gene mutations derived from clonal heterogeneity therefore represents an ideal setting for multiepitope tumor vaccination. Here, we used next generation sequencing exome resequencing to identify 962 nonsynonymous somatic point mutations in B16F10 murine melanoma cells, with 563 of those mutations in expressed genes. Potential driver mu ...[more]

PMID: 22237626

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B16-F10 RNA-Seq

Project description:B16-F10 RNA-Seq

| PRJEB5299 | ENA

proteome analysis of Lung tissue of mice bearing B16-F10-luc-G5 melanoma tumor with sleep fragmentation and with or with out the asdmistration of GL-pp

Project description:Proteome analysis of Lung tissue of mice bearing B16-F10-luc-G5 melanoma tumor with sleep fragmentation and with or with out the asdmistration of GL-pp. The mice were randomly divided into 4 groups: control group in general condition with no further treatment (CON group), tumor group with the burden of B16-F10-luc-G5 cells (Tumor group), T+SF group with SF and the burden of B16-F10-luc-G5 cells (T+SF group), and GL-pp group with SF, tumor cells burden, and the administration of 80 mg/kg GL-pp (GL-pp group). B16-F10-luc-G5 cells (5 × 1000000 cells/100 µL per mouse) were injected into the mice through the tail vein. The lung tissue of T+SF group and GL-pp group were analyzed by the proteome.

2021-09-10 | PXD025171 | Pride

RNA-seq of B16-F10 cells cultured on the ECM of SMCs treated with siRNA and B16-F10 tumor conditioned media

Project description:The objective of this study was to profile B16-F10 cells grown in vitro on the extracelllular matrix generated by SMCs treated with siRNA and conditioned media

2017-09-19 | GSE96972 | GEO

RNA-seq profiling of cultured B16 F0, F1 and F10 cells

Project description:To contrast and compare the transcriptomes of poorly metastatic B16 sub-clones F0 and F1 to the highly metastatic sub-clone F10.

2021-08-31 | GSE182970 | GEO

Transcriptomics of PTEN knockout B16-F10 cells in C57BL/6J mice model treated with PTEN

Project description:extracellular PTEN treatment did not affect the growth of PTEN knockout B16-F10 cells cultured in vitro. , To investigate whether extracellular PTEN act on the tumor microenvironment to exert a tumor-suppressive role in vivo，molecular changes caused by PTEN treatment inside the B16-F10-PTEN tumors were monitored by RNA sequencing.

2024-07-24 | GSE249142 | GEO

Mouse melanoma cell line B16-F10 treated with G007-LK

Project description:The mouse melanoma cell line B16-F10 provided by American Type Culture Collection (ATCC® CRL-6475™) were treated with DMSO, G007-LK, WNT or G007-LK+WNT, done in triplicates for a total of 12 samples.

2021-07-01 | E-MTAB-8101 | biostudies-arrayexpress

Expression data of Met-low and Met-high B16-F10 malignant melanoma cells

Project description:B16-F10 malignant mouse melanoma cells have been frequently used as highly metastatic cells. Based on heterogenous cell surface expression of Met/HGF (hepatocyte growth factor) receptor in B16-F10 cells, the cells were divided into Met-low and Met-high cells by flow cytometry and these populations were subjected to microarray analysis. Met-low and Met-high cells showed different expression profiles in genes involved characteristics of tumors, including stem cell maintenance, pigmentation, and angiogenesis.

2016-09-25 | GSE69741 | GEO

Expression data from mouse B16-F10 cells treated with heat shock (HS), Lipofectamine 2000 (LA), and benzyl alcohol (BA)

Project description:Three types of stimuli -- heat shock, Lipofectamine 2000 and benzyl alcohol -- induce activity of some stress genes (hsp) in mouse B16-F10 cells. Besides hsp genes induction, each stimulus causes gene expression changes of different sets of genes. We used microarrays to analyze global gene expression changes in mouse B16-F10 cells treated with elevated temperature (heat shock, HS), with Lipofectamine 2000 (LA) or with 40mM benzyl alcohol (BA). In order to study how lipofection may affect cellular homeostasis, we used Affymetrix microarrays to analyze the whole transcriptome of mouse B16-F10 cells treated with Lipofectamine 2000. To find out which genes are affected, we compared the cells treated with Lipofectamine (LA1, LA2, LA3), the cells treated with 40mM benzyl alcohol (BA1, BA2, BA3), heat-shocked cells (HS1, HS2, HS3) and control, untreated cells (C1-5). RNA was extracted from cells 30 min after treatment.

2011-06-04 | E-GEOD-29743 | biostudies-arrayexpress

Expression data from mouse B16-F10 cells treated with heat shock (HS), Lipofectamine 2000 (LA), and benzyl alcohol (BA)

2011-06-04 | GSE29743 | GEO

Effect of depletion of HOIP on gene expression during B16-F10 melanoma tumor development in immunedeficinet mice.

Project description:To investigate the cooperative function LUBAC E3 ligase complex during tumor development, we established HOIP-knockout B16-F10 murine melanoma cell lines.

2024-04-01 | GSE206472 | GEO

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