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Expression data from mouse B16-F10 cells treated with heat shock (HS), Lipofectamine 2000 (LA), and benzyl alcohol (BA)

ABSTRACT: Three types of stimuli -- heat shock, Lipofectamine 2000 and benzyl alcohol -- induce activity of some stress genes (hsp) in mouse B16-F10 cells. Besides hsp genes induction, each stimulus causes gene expression changes of different sets of genes. We used microarrays to analyze global gene expression changes in mouse B16-F10 cells treated with elevated temperature (heat shock, HS), with Lipofectamine 2000 (LA) or with 40mM benzyl alcohol (BA). In order to study how lipofection may affect cellular homeostasis, we used Affymetrix microarrays to analyze the whole transcriptome of mouse B16-F10 cells treated with Lipofectamine 2000. To find out which genes are affected, we compared the cells treated with Lipofectamine (LA1, LA2, LA3), the cells treated with 40mM benzyl alcohol (BA1, BA2, BA3), heat-shocked cells (HS1, HS2, HS3) and control, untreated cells (C1-5). RNA was extracted from cells 30 min after treatment.

ORGANISM(S): Mus musculus

SUBMITTER: Michal Swierniak 

PROVIDER: E-GEOD-29743 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Liposome-based DNA carriers may induce cellular stress response and change gene expression pattern in transfected cells.

Fiszer-Kierzkowska Anna A   Vydra Natalia N   Wysocka-Wycisk Aleksandra A   Kronekova Zuzana Z   Jarząb Michał M   Lisowska Katarzyna Marta KM   Krawczyk Zdzisław Z  

BMC molecular biology 20110610

<h4>Background</h4>During functional studies on the rat stress-inducible Hspa1b (hsp70.1) gene we noticed that some liposome-based DNA carriers, which are used for transfection, induce its promoter activity. This observation concerned commercial liposome formulations (LA), Lipofectin and Lipofectamine 2000. This work was aimed to understand better the mechanism of this phenomenon and its potential biological and practical consequences.<h4>Results</h4>We found that a reporter gene driven by Hspa1  ...[more]

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