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DNA:RNA Immunoprecipitation and Tiling Microarray (DRIP-chip)

ABSTRACT: Experiment to obtain the genome-wide distribution of DNA:RNA hybrid prone loci in Saccharomyces cerevisiae by DNA:RNA immunoprecipitation and tiling microarray (DRIP-chip). Samples: wild type, Rnase H deletion mutant, hpr1 deletion mutant, sen1-1 temperature sensitive mutant.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Yujia Alina Chan

PROVIDER: E-MTAB-2388 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide profiling of yeast DNA:RNA hybrid prone sites with DRIP-chip.

Chan Yujia A YA Aristizabal Maria J MJ Lu Phoebe Y T PY Luo Zongli Z Hamza Akil A Kobor Michael S MS Stirling Peter C PC Hieter Philip P

PLoS genetics 20140417 4

DNA:RNA hybrid formation is emerging as a significant cause of genome instability in biological systems ranging from bacteria to mammals. Here we describe the genome-wide distribution of DNA:RNA hybrid prone loci in Saccharomyces cerevisiae by DNA:RNA immunoprecipitation (DRIP) followed by hybridization on tiling microarray. These profiles show that DNA:RNA hybrids preferentially accumulated at rDNA, Ty1 and Ty2 transposons, telomeric repeat regions and a subset of open reading frames (ORFs). Th ...[more]

PMID: 24743342

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