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Transcription profiling of logarithmically growing fission yeast

ABSTRACT: To estimate mRNA steady-state levels we used RNA extracted from logarithmically growing fisson yeast cells on Affymetrix Yeast 2.0 Genechip arrays. The signal intensities from two independent biological repeats were averaged, resulting in measurements for 4818 out of 4962 nuclear protein-coding genes.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Daniel Lackner

PROVIDER: E-TABM-220 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A network of multiple regulatory layers shapes gene expression in fission yeast.

Lackner Daniel H DH Beilharz Traude H TH Marguerat Samuel S Mata Juan J Watt Stephen S Schubert Falk F Preiss Thomas T Bähler Jürg J

Molecular cell 20070401 1

Gene expression is controlled at multiple layers, and cells may integrate different regulatory steps for coherent production of proper protein levels. We applied various microarray-based approaches to determine key gene-expression intermediates in exponentially growing fission yeast, providing genome-wide data for translational profiles, mRNA steady-state levels, polyadenylation profiles, start-codon sequence context, mRNA half-lives, and RNA polymerase II occupancy. We uncovered widespread and ...[more]

PMID: 17434133

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