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Strand-specific RNA-seq of nine rat tissues

ABSTRACT: This experiment is contains rat organism part samples and strand-specific RNA-seq data from experiment E-GEOD-41637 (https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41637/), which aimed at assessing tissue-specific transcriptome variation across mammals, with chicken used as an outgroup in evolutionary analyses. Each organism part was sourced from animals of three different strains (Sprague-Dawley, BN/SsNHsd and F344/Cr1). This data set was originally submitted to NCBI Gene Expression Omnibus under accession number GSE41637 (http://www.ncbi.nlm.nih.gov/projects/geo/query/acc.cgi?acc=GSE41637) and later imported to ArrayExpress as E-GEOD-41637.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jason Merkin

PROVIDER: E-MTAB-2800 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Evolutionary dynamics of gene and isoform regulation in Mammalian tissues.

Merkin Jason J Russell Caitlin C Chen Ping P Burge Christopher B CB

Science (New York, N.Y.) 20121201 6114

Most mammalian genes produce multiple distinct messenger RNAs through alternative splicing, but the extent of splicing conservation is not clear. To assess tissue-specific transcriptome variation across mammals, we sequenced complementary DNA from nine tissues from four mammals and one bird in biological triplicate, at unprecedented depth. We find that while tissue-specific gene expression programs are largely conserved, alternative splicing is well conserved in only a subset of tissues and is f ...[more]

PMID: 23258891

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