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Unknown,Transcriptomics,Genomics,Proteomics

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Transciption profiling by array of human umbilical cord blood Natural Killer cells after overnight treatment with or without IL-15

ABSTRACT: Cord blood NK cells were cultured with or without IL-15 overnight and then RNA was isolated using an RNeasy Mini kit (Qiagen). Samples were processed using GeneChip Whole Transcript Sense Target Labeling assays using the Ambion WT Expression kit and Affymetrix GeneChip WT Terminal Labeling and Controls kit (Affymetrix). The resulting ssDNAs were hybridized to the Human Gene 2.0 ST Array (Affymetrix) and transcript levels compared between IL-15 treated and untreated using GeneSpring software.

ORGANISM(S): Homo sapiens

SUBMITTER: Aurore Saudemont

PROVIDER: E-MTAB-2847 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Natural Killer Cells Improve Hematopoietic Stem Cell Engraftment by Increasing Stem Cell Clonogenicity In Vitro and in a Humanized Mouse Model.

Escobedo-Cousin Michelle M Jackson Nicola N Laza-Briviesca Raquel R Ariza-McNaughton Linda L Luevano Martha M Derniame Sophie S Querol Sergio S Blundell Michael M Thrasher Adrian A Soria Bernat B Cooper Nichola N Bonnet Dominique D Madrigal Alejandro A Saudemont Aurore A

PloS one 20151014 10

Cord blood (CB) is increasingly used as a source of hematopoietic stem cells (HSC) for transplantation. Low incidence and severity of graft-versus-host disease (GvHD) and a robust graft-versus-leukemia (GvL) effect are observed following CB transplantation (CBT). However, its main disadvantages are a limited number of HSC per unit, delayed immune reconstitution and a higher incidence of infection. Unmanipulated grafts contain accessory cells that may facilitate HSC engraftment. Therefore, the ef ...[more]

PMID: 26465138

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