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In vivo ChIP-seq analysis of Foxa1 and Foxa2 binding sites in midbrain dopaminergic progenitors and neurons

ABSTRACT: E12.5 wild-type embryos were dissected to collect ventral midbrain regions. Samples were crosslinked 10min with 1% formaldehyde and processed for chromatin extraction and Chromatin Immunoprecipitation (ChIP) following the Millipore upstate protocol. Libraries were prepared using the illumina ChIP-Seq DNA Sample Prep Kit. ChIP-Seq libraries were sequenced on the Illumina GAIIx.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Mus musculus

SUBMITTER: Kamal Bouhali

PROVIDER: E-MTAB-3332 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ChIP-seq analysis of Foxa2 binding sites in midbrain dopaminergic progenitors

Project description:Embryonic stem (ES) cells were differentiated in culture to midbrain dopaminergic (mDA) progenitors and subjected to ChIP-seq analysis to resolve genome-wide binding sites of forkhead box protein A2 (Foxa2). Foxa2 was found to directly regulate multiple lineage pathways to specify midbrain dopaminergic and floor plate progenitor identity.

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