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Transcriptional changes during Daphnia pulex development indicate that the maturation decision resembles a rate more than a threshold

ABSTRACT: Maturation is a critical developmental process, and the age and size at which it occurs have important fitness consequences. Maturation is also remarkably variable across species, genotypes and environments. Despite this variation, certain key mechanisms, including a minimum size or state threshold for maturation, are proposed to underlie maturation across a broad diversity of taxa. Physiological changes associated with thresholds have been elucidated in some model systems; however, there is evidence that thresholds are not fixed but developmentally plastic. In the crustacean Daphnia pulex, it is unclear whether maturation follows a threshold or is a gradual process more akin to a rate. Changes in gene expression across four instars before and during maturation were compared in a cDNA microarray experiment. In total 45 genes were significantly differentially expressed over the course of ontogeny. Among those showing higher levels of expression later in development were genes for lipid-transport proteins (six), histones (eight) and involved in RNA processing or transcription (five). Eight of the 45 genes, including several vitellogenins, showed a marked increase in expression between developmental stages, which may explain the maturation threshold previously posited in this species. However, most genes showed gradual increases or decreases in expression during the course of development, suggesting a maturation process more analogous to a rate than a discrete developmental switch.

ORGANISM(S): Daphnia pulex

SUBMITTER: Ewan Harney

PROVIDER: E-MTAB-3388 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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