Unknown,Transcriptomics,Genomics,Proteomics

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Lysate affects on keratinocyte scratch assays


ABSTRACT: The aim of this study was to provide proof of principle as to whether probiotic bacteria or their extracts, could stimulate cutaneous wound healing. To this end, we have used a keratinocyte monolayer scratch assay which assesses one important aspect of wound healing, re-epithelialization. Primary human keratinocyte monolayers were scratched and then exposed to lysates of Lactobacillus rhamnosus GG

ORGANISM(S): Homo sapiens

SUBMITTER: Leo Zeef 

PROVIDER: E-MTAB-3485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Lactobacillus rhamnosus GG Lysate Increases Re-Epithelialization of Keratinocyte Scratch Assays by Promoting Migration.

Mohammedsaeed Walaa W   Cruickshank Sheena S   McBain Andrew J AJ   O'Neill Catherine A CA  

Scientific reports 20151105


A limited number of studies have investigated the potential of probiotics to promote wound healing in the digestive tract. The aim of the current investigation was to determine whether probiotic bacteria or their extracts could be beneficial in cutaneous wound healing. A keratinocyte monolayer scratch assay was used to assess re-epithelialization; which comprises keratinocyte proliferation and migration. Primary human keratinocyte monolayers were scratched then exposed to lysates of Lactobacillu  ...[more]

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