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Identification the proteins interacted with ILF2 in human keratinocytes

ABSTRACT: By profiling lncRNA expression changes during human skin wound healing and screening lncRNA functions, we identified SNHG26 as a pivotal regulator in keratinocyte progenitors underpinning this phase transition. we performed RNA pull-down assay in human keratinocyte progenitors and found SNHG26 interact with ILF2. ILF2, or the nuclear condensates it forms, is crucial for maintaining the stability of SNHG26. To identify other protein components within these condensates, we employed a proximity-dependent biotin identification (BioID) assay, which label proteins in close proximity to ILF2 that fused to BioID2, and subsequently identifying them through mass spectrometry (MS).

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte, Skin

DISEASE(S): Wounds And Injuries

SUBMITTER: Lihua Luo

LAB HEAD: Dongqing Li

PROVIDER: PXD055235 | Pride | 2024-08-28

REPOSITORIES: pride

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			Action	DRS
	Ctrl_S1-B3_1_7841.d.zip	Other
	ILF2_BIOID2_S1-B2_1_7840.d.zip	Other
	ILF2_BioID_followed_by_MS.xlsx.zip	Xlsx
	checksum.txt	Txt

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