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The effect of Dis3 knockdown on miRNA expression in Drosophila wing imaginal discs.

ABSTRACT: We investigated the effect on miRNA expression in Drosophila melanogaster wing imaginal discs following the knockdown of the 3'-5' exoribonuclease Dis3.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Benjamin Towler

PROVIDER: E-MTAB-3569 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The 3'-5' exoribonuclease Dis3 regulates the expression of specific microRNAs in Drosophila wing imaginal discs.

Towler Benjamin P BP Jones Christopher I CI Viegas Sandra C SC Apura Patricia P Waldron Joseph A JA Smalley Sarah K SK Arraiano Cecilia M CM Newbury Sarah F SF

RNA biology 20150101 7

Dis3 is a highly conserved exoribonuclease which degrades RNAs in the 3'-5' direction. Mutations in Dis3 are associated with a number of human cancers including multiple myeloma and acute myeloid leukemia. In this work, we have assessed the effect of a Dis3 knockdown on Drosophila imaginal disc development and on expression of mature microRNAs. We find that Dis3 knockdown severely disrupts the development of wing imaginal discs in that the flies have a "no wing" phenotype. Use of RNA-seq to quan ...[more]

PMID: 25892215

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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