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Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding

ABSTRACT: We investigated the effect that intragenic s54 binding sites have on transcripton elongation. In an rpoN deletion we have over-expressed rpoN from a plasmid (with vector only contol) and then looked at changes in transcription elongation around intragenic s54 binding sites by RNA-seq. We are also looking at changes in transcription initiation/elongation at sites of sigma factor overlap.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Escherichia coli

SUBMITTER: Carol Smith

PROVIDER: E-MTAB-3699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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