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RNA-Seq of wild type E. coli MG1655 versus those with a deletion in the hns gene (Histone-like protein H-NS)

ABSTRACT: The aim of the experiment was to determine differences in RNA levels between wild-type E. coli and E. coli with an hns deletion. We used RNA-seq, but to control for variation in sample preparation and sequencing, we combined each E. coli RNA sample with a spike-in Salmonella RNA sample of known concentration. Libraries constructed in duplicate from strains grown with and without Salmonella spike-in to assess absolute genome-wide changes in transcription.This allowed us to normalise RNA levels between samples during data analysis.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Anne Stringer

PROVIDER: E-MTAB-4751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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