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Protein occupancy of Mycoplasma pneumoniae chromosome

ABSTRACT: Protein occupancy is a means of identifying all the regions in the genome that are bound to proteins. A simple protocol is used whereby cross-linked chromatin is digested with DNase to remove all non-crosslinked, naked, DNA. To distinguish the regions that are bound by RNA polymerase from the rest, a ChAPseq (chromatin affinity precipitation) with TAP-tagged alpha subunit of RNApol, was performed.

INSTRUMENT(S): Illumina HiSeq 2500, Illumina Genome Analyzer II

ORGANISM(S): Mycoplasma pneumoniae M129

SUBMITTER: Eva Yus

PROVIDER: E-MTAB-3783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacterium.

Junier Ivan I Unal E Besray EB Yus Eva E Lloréns-Rico Verónica V Serrano Luis L

Cell systems 20160526 6

Coordination of transcription in bacteria occurs at supra-operonic scales, but the extent, specificity, and mechanisms of such regulation are poorly understood. Here, we tackle this problem by profiling the transcriptome of the model organism Mycoplasma pneumoniae across 115 growth conditions. We identify three qualitatively different levels of co-expression corresponding to distinct relative orientations and intergenic properties of adjacent genes. We reveal that the degree of co-expression bet ...[more]

PMID: 27237741

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