Unknown,Transcriptomics,Genomics,Proteomics

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Sheep Tissue Atlas

ABSTRACT: Sheep total RNA was extracted from embryonic and adult tissues. Sequencing libraries were prepared from the RNA using the Illumina TruSeq stranded total RNA with the Ribo Zero gold option for the rRNA removal. The fragmentation in the standard protocol was modified to increase the average insert size in the library. Sequencing with 151 base paired end reads was performed on an Illumina HiSeq 2500 in rapid mode.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Ovis aries

SUBMITTER: Frances Turner 

PROVIDER: E-MTAB-3838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The sheep genome illuminates biology of the rumen and lipid metabolism.

Jiang Yu Y   Xie Min M   Chen Wenbin W   Talbot Richard R   Maddox Jillian F JF   Faraut Thomas T   Wu Chunhua C   Muzny Donna M DM   Li Yuxiang Y   Zhang Wenguang W   Stanton Jo-Ann JA   Brauning Rudiger R   Barris Wesley C WC   Hourlier Thibaut T   Aken Bronwen L BL   Searle Stephen M J SMJ   Adelson David L DL   Bian Chao C   Cam Graham R GR   Chen Yulin Y   Cheng Shifeng S   DeSilva Udaya U   Dixen Karen K   Dong Yang Y   Fan Guangyi G   Franklin Ian R IR   Fu Shaoyin S   Guan Rui R   Highland Margaret A MA   Holder Michael E ME   Huang Guodong G   Ingham Aaron B AB   Jhangiani Shalini N SN   Kalra Divya D   Kovar Christie L CL   Lee Sandra L SL   Liu Weiqing W   Liu Xin X   Lu Changxin C   Lv Tian T   Mathew Tittu T   McWilliam Sean S   Menzies Moira M   Pan Shengkai S   Robelin David D   Servin Bertrand B   Townley David D   Wang Wenliang W   Wei Bin B   White Stephen N SN   Yang Xinhua X   Ye Chen C   Yue Yaojing Y   Zeng Peng P   Zhou Qing Q   Hansen Jacob B JB   Kristensen Karsten K   Gibbs Richard A RA   Flicek Paul P   Warkup Christopher C CC   Jones Huw E HE   Oddy V Hutton VH   Nicholas Frank W FW   McEwan John C JC   Kijas James J   Wang Jun J   Worley Kim C KC   Archibald Alan L AL   Cockett Noelle N   Xu Xun X   Wang Wen W   Dalrymple Brian P BP  

Science (New York, N.Y.) 20140601 6188

Sheep (Ovis aries) are a major source of meat, milk, and fiber in the form of wool and represent a distinct class of animals that have a specialized digestive organ, the rumen, that carries out the initial digestion of plant material. We have developed and analyzed a high-quality reference sheep genome and transcriptomes from 40 different tissues. We identified highly expressed genes encoding keratin cross-linking proteins associated with rumen evolution. We also identified genes involved in lip  ...[more]

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