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Rice transcriptome project

ABSTRACT: Thus, our observations suggested an intriguing possibility that OsCERK1 functions as the receptor of the mycorrhizal signal Myc-LCOs, although the function of Myc-LCOs has been examined only in dicotyledonous plants. To test this hypothesis, we evaluated Myc-LCO responses in rice by transcriptome analysis using next-generation sequencing.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: TOKYO_AGRITECH TOKYO_AGRITECH

PROVIDER: E-MTAB-4050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The bifunctional plant receptor, OsCERK1, regulates both chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice.

Miyata Kana K Kozaki Toshinori T Kouzai Yusuke Y Ozawa Kenjirou K Ishii Kazuo K Asamizu Erika E Okabe Yoshihiro Y Umehara Yosuke Y Miyamoto Ayano A Kobae Yoshihiro Y Akiyama Kohki K Kaku Hanae H Nishizawa Yoko Y Shibuya Naoto N Nakagawa Tomomi T

Plant & cell physiology 20140917 11

Plants are constantly exposed to threats from pathogenic microbes and thus developed an innate immune system to protect themselves. On the other hand, many plants also have the ability to establish endosymbiosis with beneficial microbes such as arbuscular mycorrhizal (AM) fungi or rhizobial bacteria, which improves the growth of host plants. How plants evolved these systems managing such opposite plant-microbe interactions is unclear. We show here that knockout (KO) mutants of OsCERK1, a rice re ...[more]

PMID: 25231970

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Gene expression response of Medicago truncatula roots treated with symbiotic lipochitooligosaccharides (LCOs)

2012-05-31 | GSE33636 | GEO

Medicago truncatula Transcriptome or Gene expression

Project description:Our global transcriptomic approach to study Myc-LCO signalling in M. truncatula used an experimental design of three genotypes â A17 (WT), dmi3 and nsp1 â and three treatments â sulphated Myc-LCOs (S Myc-LCOs), nonsulphated Myc-LCOs (NS Myc-LCOs), and solvent controls (acetonitrile, ACN). We pooled two early treatment time points (2 + 4 h) based on preliminary data and reports of transcriptional changes occurring within hours of Myc-LCO treatment. Three biological replicates were sequenced per condition. We used M. truncatula dmi3 and nsp1 mutants, described originally as NodâMycâ and NodâMyc+, respectively, and the two major types of Myc-LCOs (sulphated and nonsulphated) to determine overlaps and specificities of Myc-LCO responses. Myc-LCOs were used at 10â6 M because they are biologically active at about a 100-fold higher concentration than Nod factors, which have been used at 10â7, 10â8 M in transcriptomic studies.

2014-06-24 | E-MTAB-5182 | biostudies-arrayexpress

Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2

Project description:Many plants associate with arbuscular mycorrhizal fungi for nutrient acquisition, while legumes also associate with nitrogen-fixing rhizobial bacteria. Both associations rely on symbiosis signaling and here we show that cereals can perceive lipochitooligosaccharides (LCOs) for activation of symbiosis signaling, surprisingly including Nod factors produced by nitrogen-fixing bacteria. However, legumes show stringent perception of specifically decorated LCOs, that is absent in cereals. LCO perception in plants is activated by nutrient starvation, through transcriptional regulation of Nodulation Signaling Pathway (NSP)1 and NSP2. These transcription factors induce expression of an LCO receptor and act through the control of strigolactone biosynthesis and the karrikin-like receptor DWARF14-LIKE. We conclude that LCO production and perception is coordinately regulated by nutrient starvation to promote engagement with mycorrhizal fungi. Our work has implications for the use of both mycorrhizal and rhizobial associations for sustainable productivity in cereals.

2022-10-06 | GSE214698 | GEO

Gene expression data from Medicago truncatula plantlet roots treated with symbiotic lipochitooligosaccharides (LCOs).

Project description:Legumes interact with soil microbes, leading to the development of nitrogen-fixing root nodules and arbuscular mycorrhizal (AM) roots. While nodule initiation by diffusible lipochitooligosaccharide (LCO) Nod-factors of bacterial origin (Nod-LCOs) is well characterized, diffusible AM fungal signals were only recently identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs in parallel to Nod-LCOs, we used GeneChips to detail the global programme of gene expression in response to the external application of symbiotic LCOs. To harvest tissues for transcriptome profiling, three biological replicates consisting of 20 plantlets per treatment were selected. After 6 h of incubation in the climate chamber, 10 plantlets per batch were removed from the treatment (Myc-LCOs or Nod-LCOs) or control solutions and harvested, while the other 10 remained in the respective solutions for a total of 24 h. During harvest, one mm of the root tip of each plantlet was removed and discarded. The remaining 2 to 2.5 cm of the distal root region were cut off and directly frozen in liquid nitrogen.

2012-05-30 | E-GEOD-33636 | biostudies-arrayexpress

Gene expression response in roots of Medicago truncatula mutants treated with Myc LCOs

2012-05-31 | GSE33637 | GEO

Gene expression response in roots of Medicago truncatula mutants treated with Myc-LCOs

2015-11-12 | GSE67166 | GEO

Gene expression data from Medicago truncatula mutant plantlet roots treated with Myc-LCOs.

Project description:Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs. To harvest tissues for transcriptome profiling, three biological replicates consisting of 10 plantlets per treatment were selected. After 6 h of incubation in the climate chamber, 10 plantlets per batch were removed from the treatment (Myc-LCOs) or control solutions and harvested. During harvest, one mm of the root tip of each plantlet was removed and discarded. The remaining 2 to 2.5 cm of the distal root region were cut off and directly frozen in liquid nitrogen.

2012-05-30 | E-GEOD-33637 | biostudies-arrayexpress

Cryobiopsy: A Breakthrough Strategy for Clinical Utilization of Lung Cancer Organoids

Project description:The biggest problem with lung cancer organoid (LCO) is that most lung cancer organoids are derived from surgical specimens of early-stage lung cancer patients. In fact, patients who initially need chemotherapy are patients with advanced lung cancer who cannot undergo a surgery. So, making lung organoids from biopsy specimens successfully is an urgent task. The success rate of culturing LCO from biopsy tissues is very low because conventional lung biopsies such as transthoracic needle biopsy and forcep biopsy only get a small amount of lung tissue. The possibility of critical complications such as bleeding and pneumothorax makes it difficult to obtain enough specimens. Overgrowth of normal lung cells in late passages is also a critical problem in LCO and optimized culture conditions for LCO remain to be identified. To overcome the hurdles of lung cancer organoid, we made LCOs from cryobiopsy specimens of all stages of lung cancer patients. Transbronchial cryobiopsy can obtain over 10 times greater volume of tissue compared to bronchoscopic forceps biopsy and it can be applied to even peripheral lesions with radial endobronchial ultrasonography. The success rate of LCO culture was also markedly improved and recapitulated the characteristics of primary tumors. LCOs derived from cryobiopsy specimens can overcome the critical limitations of present lung cancer organoids. We expect that cryobiopsy will be a breakthrough strategy of clinical application of LCO in all stages of lung cancer.

2023-07-07 | GSE230537 | GEO

Alterations in soybean gene expression profile after foliar application of lipo-chitooligosaccharide (LCO) from Bradyrhizobium japonicum under sub-optimal temperature

Project description:Lipo-chitooligosaccharides (LCOs) produced by N2-fixing rhizobacteria initiate host nodule formation. Foliar application of LCOs has been shown to induce stress-related genes under optimal growth conditions. To study the effects of LCO foliar spray under stressed conditions, soybean seedlings grown at optimal temperature were exposed to sub-optimal temperature. After a 5-day acclimation period, the first trifoliolate leaves were sprayed with 10-7 M LCO (NodBj-V (C18:1, MeFuc)) produced by Bradyrhizobium japonicum, and harvested at 0 and 48 h following treatment. Microarray analysis was performed using Affymetrix GeneChip® Soybean Genome Arrays. A total of 147 genes were differentially expressed 48 h after LCO treatment, including a number of stress-related genes and transcription factors. In addition, during the 48 h following treatment, hundreds of genes were differentially expressed in LCO-treated plants, indicating that the dynamic soybean foliar transcriptome was highly responsive to LCO treatment. The microarray data was supported by quantitative real-time PCR data.

2010-03-20 | GSE20972 | GEO

Alterations in soybean gene expression profile after foliar application of lipo-chitooligosaccharide (LCO) from Bradyrhizobium japonicum under sub-optimal temperature

Project description:Lipo-chitooligosaccharides (LCOs) produced by N2-fixing rhizobacteria initiate host nodule formation. Foliar application of LCOs has been shown to induce stress-related genes under optimal growth conditions. To study the effects of LCO foliar spray under stressed conditions, soybean seedlings grown at optimal temperature were exposed to sub-optimal temperature. After a 5-day acclimation period, the first trifoliolate leaves were sprayed with 10-7 M LCO (NodBj-V (C18:1, MeFuc)) produced by Bradyrhizobium japonicum, and harvested at 0 and 48 h following treatment. Microarray analysis was performed using Affymetrix GeneChipÂ® Soybean Genome Arrays. A total of 147 genes were differentially expressed 48 h after LCO treatment, including a number of stress-related genes and transcription factors. In addition, during the 48 h following treatment, hundreds of genes were differentially expressed in LCO-treated plants, indicating that the dynamic soybean foliar transcriptome was highly responsive to LCO treatment. The microarray data was supported by quantitative real-time PCR data. Soybean seedlings grown at optimal temperature (25 Â°C) were exposed to sub-optimal temperature (15 Â°C). After a 5-day acclimation period, the first trifoliolate leaves were sprayed with 10-7 M LCO (NodBj-V (C18:1, MeFuc)) produced by Bradyrhizobium japonicum, and harvested at 0 and 48 h following treatment. Total RNA was extracted and microarray analysis was performed using Affymetrix GeneChipÂ® Soybean Genome Arrays.

2010-03-20 | E-GEOD-20972 | biostudies-arrayexpress

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