Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of Lotus japonicus wildtype and Ljgln2-2 mutant plants to investigate photorespiration

ABSTRACT: Wildtype and mutant plants lacking the plastidic isoform of glutamine synthetase were grown under CO2-enriched atmosphere and then transferred to low CO2 conditions. Transcriptomic profiling was carried out for plants under CO2-enriched atmosphere and plants after 2 days of transfer to low CO2 conditions.

ORGANISM(S): Lotus japonicus

SUBMITTER: Marco Betti

PROVIDER: E-MEXP-3603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Transcriptomic and metabolic changes associated with photorespiratory ammonium accumulation in the model legume Lotus japonicus.

Pérez-Delgado Carmen M CM García-Calderón Margarita M Sánchez Diego H DH Udvardi Michael K MK Kopka Joachim J Márquez Antonio J AJ Betti Marco M

Plant physiology 20130606 4

The transcriptomic and metabolic consequences of the lack of plastidic glutamine (Gln) synthetase in the model legume Lotus japonicus were investigated. Wild-type and mutant plants lacking the plastidic isoform of Gln synthetase were grown in conditions that suppress photorespiration and then transferred for different lengths of time to photorespiratory conditions. Transcript and metabolite levels were determined at the different time points considered. Under photorespiratory active conditions, ...[more]

PMID: 23743713

Similar Datasets

Transcription profiling by array of Lotus japonicus leaves from wild type and Ljgln2-2 mutant plants exposed to drought.

Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard (University Aarhus, Denmark) and then self-propagated at the University of Seville. Ljgln2-2 mutant was isolated from photorespiratory mutant screening as described previously (Orea et al., 2002; Mï¿½rquez et al., 2005; Betti et al., 2006). The mutant progeny of two consecutive back-crosses with WT was used for the present work. WT and mutant seeds were scarified and surface-sterilized, germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture of vermiculite and sand as solid support. Five seedlings were planted in each pot and grown during 35 days in a growth chamber under 16/8 hours day/night, 20/18ï¿½C, with a photosynthetic photon flux density of 250 ï¿½mol/m2ï¿½s and a constant humidity of 70%. CO2 was automatically injected to a final concentration of 0.7% (v/v), to allow for normal growth of Ljgln2-2 mutant in a photorespiration suppressed atmosphere. Plants were watered with Hornum nutrient solution (Handberg & Stougaard, 1992). Drought was applied withholding irrigation for the reported period of time and sample plants or leaves were harvested for further analysis.

2011-04-29 | E-MEXP-2690 | biostudies-arrayexpress

Transcriptomics of drougth stress in L. japonicus under normal air conditions

Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard (University Aarhus, Denmark) and then self-propagated at the University of Seville. Seeds were scarified and surface-sterilized, germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture of vermiculite and sand as solid support. Five seedlings were planted in each pot and grown during 35 days in a growth chamber under 16/8 hours day/night, 20/18M-oM-?M-=C, with a photosynthetic photon flux density of 250 M-oM-?M-=mol/m2M-oM-?M-=s and a constant humidity of 70%. Plants were watered with Hornum nutrient solution. Drought was applied withholding irrigation for the reported period of time and sample plants or leaves were harvested for further analysis.

2012-10-06 | E-MEXP-3710 | biostudies-arrayexpress

Transcription profiling of Lotus japonicus grown under different nitrogen sources

Project description:Transcriptomic profiling was carried out for leaves of Lotus japonicus plants grown with different mineral nitrogen sources (NO3-, NH4+ or NH4NO3) or under conditions of biological nitrogen fixation (Nod). Nodulated plants were inoculated with Mesorhizobium loti and watered with nitrogen-free âHornumâ medium supplemented with 3 mM KCl. Plants under different nitrogen nutritions were watered with âHornumâ nutrient solution containing 10 mM KNO3 (NO3- plants) or with 10 mM NH4Cl supplemented with 3 mM KCl (NH4+ plants) or with 5 mM NH4NO3 supplemented with 3 mM KNO3 (NH4NO3 plants). After all the plants reached the size of 7 trifoils, leaf tissue was harvested. Every harvest involved at least three independent biological replicates for each treatment.

2016-05-17 | E-MTAB-4177 | biostudies-arrayexpress

Transcrition profiling of shoots from related model and cultivated Lotus plant species following salt acclimation

Project description:Many plant researchers have applied genomic tools to model species to identify abiotic stress responsive genes that might be useful for improving stress tolerance in crops. However, it is unclear whether this translational approach will be successful given the complexity of abiotic stress tolerance. We carried out a functional genomic (ionomic, transcriptomic and metabolomic) comparison of three model and three forage species of the genus Lotus with varying tolerance to salinity. Transcriptome analysis showed that about 60 % of expressed genes were responsive to salt treatment in one or more of the six species tested, but less than 1 % was responsive in all genotypes. Therefore, genotype-specific responses overshadowed conserved transcriptional responses to salinity and represent an impediment to translational genomics. Fortunately, 'triangulation' from multiple species enabled the identification of a core set of conserved and tolerant-specific responses that could provide durable tolerance across genotypes.

2010-08-10 | E-MEXP-2344 | biostudies-arrayexpress

Transcription profiling of Lotus japonicus grown under different salt acclimation regimes

Project description:Model legume Lotus japonicus was subjected to non-lethal long-term salinity and profiled at the transcriptomic level. Three independent experiments were performed, testing two experimental designs: a traditional gradual acclimation following a step-wise increase of salt concentration and an initial acclimation approach (ia).

2008-07-17 | E-MEXP-1204 | biostudies-arrayexpress

Transcription profiling of Legionella longbeachae in exponential and postexponential phases

Project description:To know the expression pattern in exponential and postexponential phases of Legionella longbeachae growth curve

2010-02-25 | E-MTAB-199 | biostudies-arrayexpress

TAS002

Project description:Teresa Alves dos Santos: En1 -/- mouse midbrain E13.5 against pooled control +/+ littermates

2013-08-07 | E-TABM-1104 | biostudies-arrayexpress

FJ001

Project description:RNA was isolated from dissected ventral midbrains of E14.5 Pitx3-/- and Pitx3+/+ mouse embryos. 3 Experimental samples each consisting of 3 Pitx3-/- ventral midbrains were hybridized to reference RNA derived from 10 Pitx3+/+ ventral midbrains

2011-01-12 | E-TABM-1209 | biostudies-arrayexpress

Transcription profiling of matching human primary head and neck squamous cell carcinomas and cervical lymph node metastases.

Project description:An experiment was performed to investigate the perservation of gene expression upon metastasis of primary head and neck squamous cell carcinomas to the cervical lymph node.

2006-10-23 | E-TABM-114 | biostudies-arrayexpress

Transcription profiling of Candida albicans wild type and sch9 mutant grown under normoxia, hypoxia and hypoxia with CO2

Project description:sch9-mutant and wildtype grown under normoxi, hypoxia (four hours under 0,2% O2), or hypoxia with CO2 (0,2 % O2 and 6 % CO2)

2010-03-01 | E-MEXP-2407 | biostudies-arrayexpress