Transcription profiling by array of Lotus japonicus leaves from wild type and Ljgln2-2 mutant plants exposed to drought.

ABSTRACT: L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard
(University Aarhus, Denmark) and then self-propagated at the University of Seville.
Ljgln2-2 mutant was isolated from photorespiratory mutant screening as described
previously (Orea et al., 2002; M�rquez et al., 2005; Betti et al., 2006).
The mutant progeny of two consecutive back-crosses with WT was used
for the present work. WT and mutant seeds were scarified and surface-sterilized,
germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture
of vermiculite and sand as solid support.
Five seedlings were planted in each pot and grown
during 35 days in a growth chamber under 16/8 hours
day/night, 20/18�C, with a photosynthetic photon flux
density of 250 �mol/m2�s and a constant humidity of 70%.
CO2 was automatically injected to a final concentration of 0.7% (v/v),
to allow for normal growth of Ljgln2-2 mutant in a photorespiration
suppressed atmosphere. Plants were watered with Hornum nutrient solution
(Handberg & Stougaard, 1992).
Drought was applied withholding irrigation for the reported period
of time and sample plants or leaves were harvested for further analysis.

ORGANISM(S): Lotus japonicus

SUBMITTER: Marco Betti 

PROVIDER: E-MEXP-2690 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress