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RNA-Seq analysis of Arabidopsis thaliana esr1-1 mutant versus wild-type (JC66)

ABSTRACT: A GSTF8 promoter fragment fused to the luciferase reporter gene was used in a forward genetic screen for Arabidopsis mutants with up-regulated GSTF8 promoter activity and identified the esr1-1 (enhanced stress response 1) mutant which also conferred increased resistance to the fungal pathogen Fusarium oxysporum. RNA-Seq was conducted to compare esr1-1 and wild-type (GSTF8 promoter::luciferase reporter line JC66 in Col-0 background) transcriptomes for altered gene expression.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Louise Thatcher

PROVIDER: E-MTAB-4235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Arabidopsis KH-Domain RNA-Binding Protein ESR1 Functions in Components of Jasmonate Signalling, Unlinking Growth Restraint and Resistance to Stress.

Thatcher Louise F LF Kamphuis Lars G LG Hane James K JK Oñate-Sánchez Luis L Singh Karam B KB

PloS one 20150518 5

Glutathione S-transferases (GSTs) play important roles in the protection of cells against toxins and oxidative damage where one Arabidopsis member, GSTF8, has become a commonly used marker gene for early stress and defense responses. A GSTF8 promoter fragment fused to the luciferase reporter gene was used in a forward genetic screen for Arabidopsis mutants with up-regulated GSTF8 promoter activity. This identified the esr1-1 (enhanced stress response 1) mutant which also conferred increased resi ...[more]

PMID: 25985302

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