Transcriptional profiling of mouse oesophageal dysplasias
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ABSTRACT: We performed microarray analysis to assess changes in gene expression in dysplastic lesions in the mouse oesophagus versus adjacent normal epithelium. To induce tumor formation, animals were treated with the tobacco-derived carcinogen DEN (diethylnitrosamine) in drinking water 3 times a week for 56 days. Subsequently, Sorafenib was administered by ip injection as a solution at 10 mg/ml for 28 days. RNA was isolated from 9 HGD (high grade dysplasia) samples and 9 control samples of adjacent normal epithelium from the same animal (n= 9 samples from a total of 6 animals). Hybridisation was performed on a Mouse Whole Genome-6 v2 Illumina array.
INSTRUMENT(S): Illumina bead array reader, Illumina TotalPrep RNA Amplification kit, Mouse Whole Genome-6 v2 Illumina array
ORGANISM(S): Mus musculus
SUBMITTER: Julia Frede
PROVIDER: E-MTAB-4244 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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