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Transcriptional profiling of mouse oesophageal dysplasias

ABSTRACT: We performed microarray analysis to assess changes in gene expression in dysplastic lesions in the mouse oesophagus versus adjacent normal epithelium. To induce tumor formation, animals were treated with the tobacco-derived carcinogen DEN (diethylnitrosamine) in drinking water 3 times a week for 56 days. Subsequently, Sorafenib was administered by ip injection as a solution at 10 mg/ml for 28 days. RNA was isolated from 9 HGD (high grade dysplasia) samples and 9 control samples of adjacent normal epithelium from the same animal (n= 9 samples from a total of 6 animals). Hybridisation was performed on a Mouse Whole Genome-6 v2 Illumina array.

INSTRUMENT(S): Illumina bead array reader, Illumina TotalPrep RNA Amplification kit, Mouse Whole Genome-6 v2 Illumina array

ORGANISM(S): Mus musculus

SUBMITTER: Julia Frede

PROVIDER: E-MTAB-4244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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