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Arabidopsis thaliana strain:ColTranscriptome or Gene expression

ABSTRACT: This study aims to identify in planta transcripts physically associated with alternative splicing regulator SR45. Interactions of SR45 with both RNAs and proteins are crucial for regulating RNA processing. However, in vivo RNA targets of SR45 are currently unclear. Using RNA immunoprecipitation followed by high-throughput sequencing, we identified over 4000 Arabidopsis thaliana RNAs that directly or indirectly associate with SR45, designated as SR45-associated RNAs

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Anireddy Reddy

PROVIDER: E-MTAB-4283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome-Wide Identification of RNA Targets of Arabidopsis SERINE/ARGININE-RICH45 Uncovers the Unexpected Roles of This RNA Binding Protein in RNA Processing.

Xing Denghui D Wang Yajun Y Hamilton Michael M Ben-Hur Asa A Reddy Anireddy S N AS

The Plant cell 20151124 12

Plant SR45 and its metazoan ortholog RNPS1 are serine/arginine-rich (SR)-like RNA binding proteins that function in splicing/postsplicing events and regulate diverse processes in eukaryotes. Interactions of SR45 with both RNAs and proteins are crucial for regulating RNA processing. However, in vivo RNA targets of SR45 are currently unclear. Using RNA immunoprecipitation followed by high-throughput sequencing, we identified over 4000 Arabidopsis thaliana RNAs that directly or indirectly associate ...[more]

PMID: 26603559

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