Triticum aestivum strain:Wangshuibai Transcriptome or Gene expression

ABSTRACT: To get an overview of transcriptome characteristics of Wangshuibai during infection by Fg, a high-throughput RNA sequencing based on next generation sequencing (NGS) technology (Illumina) were performed. Each spike of 4 central spikelets was injected with 10 μl of conidial inoculant (105 macro conidia per milliliter) and covered with a plastic bag to maintain humidity until sampling. Both non-inoculated spikes and inoculated spikes at 12, 24, 48, 72 and 96 hours after inoculation (hai) were sampled. Inoculations and sampling were conducted at 7 a.m. except for the sample at 12 hai at 7 p.m. A total of 12 samples (one treatment, two genotypes and six time points) were prepared for RNA extraction. The samples for transcriptome analysis were the mixture of equal amount of RNA from non-inoculated spikes and spikes at 12, 24 and 48 hai of Wangshuibai. Transcriptome library with fragments between 200 to 700 bp was prepared following the Illumina’s kits provided by manufacturer and sequenced on Illumina HiSeq™ 2000 using paired-end technology in a single run.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Triticum aestivum

SUBMITTER: Jin Xiao 

PROVIDER: E-MTAB-4384 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress